中国中西医结合杂志2012，Vol.32Issue(11)：1496-1499,4.
基于表面增强激光解析离子化飞行时间质谱技术的慢性肾衰竭中医湿证尿液中相关蛋白研究
Study on Correlated Proteins in the Urine of Chronic Renal Failure Patients of Chinese Medicine Damp Syndrome Based on SELDI-TOF-MS Technique
摘要
Abstract
Objective To investigate the protein markers in the urine of chronic renal failure (CRF) patients of Chinese medicine damp syndrome (CMDS) based on surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) technique. Methods The urine was sampled from 90 CRF patients of CMDS and 60 CRF patients of non-CMDS. Then the proteomics of the urine was studied by H4 gene chip. The chips were scanned and analyzed using PBS || (a protein chip reader). Results (1) Totally 25 differential protein peaks were identified in the e/m range of 1 000 -20 000 of the protein atlas of the two groups (P < 0. 01). (2) The urine protein predictive model of CFR patients of CMDS was established after bioinformatic analysis. Totally 4 biomarkers consisting of M/Z 8 654. 96, M/Z 2 081. 65, M/Z 18 667. 3, and M/Z 2 242. 14 were obtained, which could better classify the samples of CMDS and those of non-CMDS. Its accuracy rate was 84. 7% , the sensitivity was 92. 2% , and the specificity was 73. 3%. (3) Between the CMDS group and the non-CMDS group, 7 kinds of proteins in the urine were possibly identified by SwissProt Database. Conclusions This study had primarily screened the protein markers in the urine of CRF patients of CMDS, and established the predictive model. The protein markers in the urine were identified by database, thus providing certain experimental evidence for clinical typing of CRF patients of CMDS.关键词
慢性肾衰竭/中医湿证/尿液/表面增强激光解析离子化飞行时间质谱Key words
chronic renal failure/ Chinese medicine damp syndrome/ urine/ surface-enhanced laser desorption/ionization time of flight mass spectrometry引用本文复制引用
郝一鸣,洪名超,王文静,金亚明,王忆勤..基于表面增强激光解析离子化飞行时间质谱技术的慢性肾衰竭中医湿证尿液中相关蛋白研究[J].中国中西医结合杂志,2012,32(11):1496-1499,4.基金项目
上海市教委科研创新项目(No.08ZZ63) （No.08ZZ63）
国家中医药管理局中医诊断学重点学科(No.S30302) （No.S30302）
