中国中西医结合杂志2012，Vol.32Issue(11)：1516-1520,5.
疏肝健脾方对实验性腹泻型肠易激综合征5-羟色胺
Effects of Shugan Jianpi Recipe on the Ion Transport of Diarrhea Predominant Irritable Bowel Syndrome Rat Colon Mucosa Induced by 5-HT
摘要
Abstract
Objective To investigate the mechanism of Shugan Jianpi Recipe (SJR) on the ion transportation of diarrhea predominant irritable bowel syndrome (IBS-D) colon mucosa induced by 5-HT. Methods Totally 36 male SD rats were randomly divided into three groups, i. e. , the normal group, the model group, and the SJR group, 12 in each group. IBS-D Rat model was induced by intracolonic instillation of acetic acid and restraint stress. After successful modeling, normal saline was given to rats in the normal group and the model group, while SJR was given to those in the SJR group by gastrogavage for 14 days. The short circuit current (Isc) technology was used to measure 5-HT induced Isc changes of the colon mucosa under the actions of drugs and specific blocking agents. Results There was no difference in basal current (BC), the potential difference (PD), and transmembrane resistance (TR) of the distal colon among the 3 groups (all P>0. 05). The 5-HT induced short circuit current change ( Alsc) was lower in the model group than in the normal group (P<0. 05), and it was higher in the SJR group than in the model group (P<0. 05). When 4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid (DIDS, 500 μmol/L), an Ca + -dependent Cl'channel blocker, was added from the epiphragm of the colonic mucosa, the 5-HT induced Alsc was lower in the model group than in the normal group (P<0. 05), and it was higher in the SJR group than in the model group ( P < 0. 05). When Na+ was substituted in the epiphragm solution (Na+free) or amiloride (100 μmol/l_) was added from the epiphragm of the colonic mucosa, an epithelial Na + channel blocker, the 5-HT induced Alsc was lower in the model group than in the normal group (P<0. 05), and it was higher in the SJRgroup than in the model group (P<0. 05). Conclusions SJR could affect the transmembrane electrolyte transportation of IBS-D rat induced by 5-HT through regulating the secretion of Cl'and HCO3". The effects might be a-chieved by the coordination of apical Cl'channel CFTR, basolateral Na + -K+ ATPase, sodium-potassium-chloride cotransporter, sodium-bicarbonate cotransporter, CIVHCO3"exchanger, as well as K + channel.关键词
疏肝健脾方/结肠上皮/腹泻型肠易激综合征/短路电流Key words
Shugan Jianpi Recipe/ colonic epithelium/ diarrhea predominant irritable bowel syndrome/ short circuit current引用本文复制引用
张声生,汪正芳,郭前坤,陈剑明,李琳,李萍..疏肝健脾方对实验性腹泻型肠易激综合征5-羟色胺[J].中国中西医结合杂志,2012,32(11):1516-1520,5.基金项目
国家自然科学基金资助项目(No.30973746) （No.30973746）
北京市自然科学基金资助项目(No.7102083) （No.7102083）
北京市教委科技发展计划资助项目(No.KM201010025027) （No.KM201010025027）
