中药材2012，Vol.35Issue(6)：886-892,7.
白花蛇舌草药材的HPLC指纹图谱研究
Study on HPLC Fingerprint of Oldenlandia diffusa
摘要
Abstract
Objective:To establish the HPLC fingerprint chromatogram of Oldenlandia diffusa coupled with chemometrics means for the quality control of multi-batches of medicinal material. Methods:The separation was developed on C18 column(4. 6 mm ×250 mm, 5 μm) by gradient elution with acetonitrile-water(both containing 0. 1‰( V/V) ocetic acid) as mobile phase at a flow rate of 0. 8 mL/ min, the detection wavelength at 238 nm and column temperature at 30 t. The HPLC fingerprint chromatogram of Oldenlandia diffusa was set up and the main characteristic peaks were identified by comparing with chemical reference substance. The quality of 22 batches of medicinal material was evaluated by similarity assay as well as principal component analysis (PCA) and cluster analysis. Results: The established HPLC fingerprint chromatogram of Oldenlandia diffusa was specific, precise, reproducible and stable. 11 peaks were chemically identified. The similarity of 17 batches of Oldenlandia diffusa was obviously higher than 5 batches of adulterants. PCA showed that 17 batches of Oldenlandia diffusa were in a domain and 5 batches of adulterants were far apart from the domain. The cluster analysis of the 22 batches of medicinal material showed that 17 batches of Oldenlandia diffusa were in a cluster while 5 batches of a-dulterants were excluded. Further cluster analysis was carried out for the quality consistency of 17 batches of Oldenlandia diffusa and accordingly they were devided into 4 clusters. Conclusion: With the combination of chemometrics means, the HPLC fingerprint chromatogram provides a method for evaluation of authenticity and quality control of Oldenlandia diffusa, which is favorable to improve overall quality control of Oldenlandia diffusa.关键词
白花蛇舌草/高效液相色谱法/指纹图谱/化学计量学/质量控制Key words
Oldenlandia diffusa ( Willd. ) Roxb. / HPLC/ Fingerprint/ Chemometrics/ Quality control分类
医药卫生引用本文复制引用
陈妍,姚志红,戴毅,程红,温丽荣,周光雄,姚新生..白花蛇舌草药材的HPLC指纹图谱研究[J].中药材,2012,35(6):886-892,7.基金项目
广东省自然科学基金(No.8351063201000003) （No.8351063201000003）
