中药材2012，Vol.35Issue(6)：892-896,5.
金盏银盘指纹图谱的建立及其活性成分的研究
Analysis of Fingerprint and Bioactive Components of Bidens biternata by HPLC
摘要
Abstract
Objective:To establish HPLC fingerprint of Bidens biternata from different habitats and determine the contents of hy-peroside, isoquercetin, astragalin and bipinnatapolyacetylpside. Methods; Analysis was carried on Hypersil ODS C18 column (4. 6 mm ×250 mm, 5.0 μm) with acetonitrile and 3% acetic acid as the mobile phase in a gradient elution. The contents of 4 components were determined simultaneously. Results: The fingerprint of 10 populations were established and the data were analyzed by the similarity evaluation software. There were almost no differences between the similarities of 10 population, but the contents of 4 main compoerls were different among them. Conclusion: This method is stable and reliable which could be applied in quality assessment.关键词
金盏银盘/HPLC/指纹图谱/金丝桃苷/异槲皮苷/鬼针聚炔苷/紫云英苷Key words
Bidens biternata ( Lour. ) Merr. et sherff. / HPLC/ Fingerprint/ Hyperoside/ Isoquercetin/ Astragalin/ Bipinnatapolyacetylpside分类
医药卫生引用本文复制引用
马陶陶,谢晋,张群林,许慧,李俊,陈飞虎..金盏银盘指纹图谱的建立及其活性成分的研究[J].中药材,2012,35(6):892-896,5.基金项目
国家重大新药创制专项(No.2009ZX09103386) （No.2009ZX09103386）
