中药材2012，Vol.35Issue(6)：930-935,6.
齐墩果酸对四氯化碳诱导大鼠肝硬化门静脉高压的影响
The Anti-portal Hypertension Effect of Oleanolic Acid in CCl4-induced Cirrhosis Rats
摘要
Abstract
Objective:To study the anti-portal hypertension effect of oleanolic acid(OA) in CC14-induced cirrhosis rats and its mechanism. Methods:Rats were induced to portal hypertension by CC14. After treatment with low dose of OA(30 mg/kg)and high dose of OA(60 mg/kg)by intragastrically for a month, the parameters in serum or liver tissue including ALT, AST,MDA,GSH-Px,NOx,eNOS, cGMP and type I collagen were measured. The MAP, PP and HR were determined by hameodynamic method and the eNOS expression in liver was measured by western blot. The pathological changes of liver tissue were also tested by Masson dye. The normal group and model group were given 0. 25% of CMC-Na solution. Results;Compared with the model group, treatment with 30 mg/kg and 60 mg/kg 0A significantly decreased the levels of ALT, AST,ALP,y-GT and MDA and enhanced the level of GSH-Px in liver(P <0. 05). More-over,the collagen content also notably lowered in CC14-induced cirrhosis rats,thus decreasing the portal pressure(PP). However,the MAP and HR were not affected by OA treatment In addition,the expression of eNOS in liver markedly increased after one mouth treatment of OA,hereof enhancing the level of cGMP and NOx in the CC14-induced portal hypertensive rats(P <0. 05). Conclusion: OA could inhibit the progress of fibrosis and lower the PP in CC14-induced portal hypertensive rats and the anti-portal hypertension effect might be related to increasing the expression of eNOS and enhance the NOx level in liver.关键词
齐墩果酸(OA)/门静脉高压/一氧化氮(NO)/四氯化碳/内皮型一氧化氮合酶(eNOS)Key words
Oleanolic acid/ Portal hypertension/ Nitric oxide (NO) / CC14 / Endothelial nitric oxide synthase(eNOS)分类
医药卫生引用本文复制引用
刘昌辉,黄小桃,李颖仪,郑侠,李能,宓穗卿,王宁生..齐墩果酸对四氯化碳诱导大鼠肝硬化门静脉高压的影响[J].中药材,2012,35(6):930-935,6.基金项目
广东省"211工程"三期重点学科建设项目"药物警戒系统中的中药再评价研究"专项基金 （）
2011国家自然基金资助项目(81102883) （81102883）
广东省自然科学基金资助项目(S2011010005540) （S2011010005540）
广东省优秀博士学位论文作者资助项目(AFD004112A01) （AFD004112A01）
