中药材2012，Vol.35Issue(6)：935-939,5.
半夏白术天麻汤对自发性高血压大鼠肾脏蛋白表达谱的影响
The Effect of Banxia Baizhu Tianma Decoction on Renal Protein Expression in Spontaneously Hypertensive Rats
摘要
Abstract
Objective:To study the effect of Banxia Baizhu decoction on the spontaneously hypertensive rats and its possible mechanism. Methods: Male SHRs of 6 weeks old were randomly and equally divided into two groups, the control SHR group and the group treated with BXBZTMD,the treated group were treated with BXBZTMD orally for 18 weeks,the control SHR group and normotensive WKY group were fed with the same amount of distilled water. Rats' blood pressure was measured through carotid artery catheterization, and protein of kidney tissues was analyzed by two-dimensional electrophoresis after extraction and purification. Different protein spots expressed significantly which were anlyzed by PDQuest software were then identified by MALDI-TOF/TOF MS. The protein expressions of peroxidase 2 and Cu-Zn superoxide in kidney were determined by Western blot. Results:Compared SHR with WKY, 12 protein spots were changed. BXBZTMD could adjust kidney protein expression such as Cu-Zn superoxide dismutase, peroxidase 2 and may change kidney protein expression such as the α-β-crystallin, which were closely related to oxidative stress. These discoveries of the target proteins suggested that BXBZTMD had curative effect by anti-oxidative stress. Conclusion: Oxidative stress plays an important role in the process of hypertensive renal injury. BXBZTMD can play a therapeutic role to oxidative stress. The discovery of the target protein provides a theoretical basis for the mechanism of the therapeutic effect of BXBZTMD.关键词
双向电泳/半夏白术天麻汤/自发性高血压大鼠/肾损伤/氧化应激Key words
Two-dimensional electrophoresis/ Banxia Baizhu Tianma Decoction/ Spontaneously hypertensive rats/ Hypertensive renal injury/ Oxidative stress分类
医药卫生引用本文复制引用
罗珊珊,蒋嘉烨,栗源,陆家凤,可燕..半夏白术天麻汤对自发性高血压大鼠肾脏蛋白表达谱的影响[J].中药材,2012,35(6):935-939,5.基金项目
国家科技部十一五支撑计划资助项目(2006BAI11B08) （2006BAI11B08）
