安徽农业大学学报2012，Vol.39Issue(6)：859-862,4.
小鹅瘟的诊断及其病理组织学观察
Diagnosis of gosling plague and its histopathologic observation
摘要
Abstract
Three dead goslings, which were suspected to be infected with goose parvovirus (GPV), were diagnosed by clinical observation and pathological anatomy. The results indicated that a swollen appearance appeared in the middle or lower parts of the small intestine and a typical coagulative embolus emerged and blocked the intestinal cavity. Based on the published VP3 sequence of GPV, a pair of specific primers was designed to amplify the nucleic acids of GPV by PCR, and specific bands of 331 bp in length agreed with the desired results of GPV-VP3 were amplified from three goslings liver tissues. The organization, such as duodenum, ileum, kidney, spleen and liver were analyzed by taken into 10% formaldehyde solution, paraffin embedding, HE dyeing and histologic analysis. The results showed that the fibrinous necrotic enterit could be observed in duodenum and ileum, and the cellular morphology of other tissues were good in three cases. For Wanxi white goose, degeneration, necrosis could be observed in renal epithelial cells and the congestion, and steatosis could be observed in liver tissue and cells.关键词
小鹅瘟/诊断/PCR/病理观察Key words
gosling plague/ diagnosis/ PCR/ pathological observation分类
农业科技引用本文复制引用
王浩,张烁,刘红梅,潘玲,祁克宗..小鹅瘟的诊断及其病理组织学观察[J].安徽农业大学学报,2012,39(6):859-862,4.基金项目
安徽农业大学博士启动基金和安徽省教育厅自然科学基金重点项目(KJ2012A112)共同资助. （KJ2012A112）
