安徽农业科学2012，Vol.40Issue(32)：15588-15592,15596,6.
普鲁兰短梗霉N2-3菌株碱性蛋白酶的纯化及性质研究
Purification and Characters of the Protease from Marine Yeast Aureobosidium pullulans N2-3
摘要
Abstract
[Objective] To purify alkaline protease of Aureobosidium pullulans N2-3, and study its properties. [Method] By using Sephadex G-75 and DEAE Sepharose Fast Flow to purify alkaline protease of Aureobosidium pullulans N2-3, and SDS-PAGE was carried out. The optimal reaction temperature, temperature stability, optimal reaction pH, pH stability were determined, effects of each metal ion and compound on protease activity were studied. [Result] The purification multiples and recovery rate were 2.34, 25.9% , respectively. The molecular weight of this enzyme was about 33 kDa; the optimal temperature and pH were 52 Xl and 9.0; the enzyme was activated by Mg2 * , Na * and Mn2 * at a concentration of 5.0 mmol/L and inhibited by Fe , Fe , Cu , Co , Ag and Hg . Zn , Ca , K and Ii have no effect on the protease activity. The enzyme was strongly inhibited by phenylmethylsulfonyl fluoride, and weakly inhibited by EDTA and iodoacetic acid. [Conclusion] The study will provide a theoretical basis for industrialization production of protease from Aureobosidium pullulans N2-3.关键词
蛋白酶/纯化/性质Key words
Protease/ Purification/ Character分类
农业科技引用本文复制引用
李红,王惠,尼秀媚,白桦..普鲁兰短梗霉N2-3菌株碱性蛋白酶的纯化及性质研究[J].安徽农业科学,2012,40(32):15588-15592,15596,6.基金项目
国家质检总局项目(2011IK170). （2011IK170）
