安徽农业科学2012，Vol.40Issue(32)：15593-15596,4.
曙红与鲑鱼精蛋白显色反应的研究
Study on Color Reaction of Eosin and Salmon Sperm Protein
摘要
Abstract
[Objective] The research aimed to study the color reaction of eosin (gliadin) and salmon sperm protein. [Method] Conjugation reaction of eosin (gliadin) and salmon sperm protein which occurred in the presence of polyvinyl alcohol (PVA) was studied by spectrophotometry. The color reaction conditions and the factors which could affect the sensitivity were studied,and the best reactive conditions were determined. [ Result] When reagent blank was used as a reference,the maximum absorption wavelength of the compound which included eosin and protamine sulfate was 542 nm. Absorbance at the maximum absorption wavelength showed good linear relationship with the protein concentration. [ Conclusion ] If it was used for the quantitative determination of protein, high sensitivity, good linearity, reagent stability and low toxicity could be gained. And eosin and salmon sperm protein compound wasn't easy to adhere to the vessel wall. The operation is simple,rapid and accurate.关键词
鲑鱼精蛋白/曙红/分光光度法Key words
Salmon sperm protein/ Eosin/ Spectrophotometry分类
农业科技引用本文复制引用
韩照祥,马丽丽..曙红与鲑鱼精蛋白显色反应的研究[J].安徽农业科学,2012,40(32):15593-15596,4.基金项目
国家自然科学基金资助项目(20977040) （20977040）
江苏省海洋资源开发研究院开放项目(JSIMR10E04) （JSIMR10E04）
重庆市高校微纳米材料工程与技术重点实验室2011年度开放课题(KFJJ1106) （KFJJ1106）
江苏省滩涂生物资源与环境保护重点建设实验室开放课题(JLCBE11004). （JLCBE11004）
