安徽农业科学2012，Vol.40Issue(32)：15873-15876,4.
响应面法优化食尼古丁节杆菌发酵产海藻糖合成酶的工艺
Optimization of Process Parameters of Producing Trehalose Synthase by Arthrobacter nicotinovorus Fermentation
摘要
Abstract
[Objective] To optimize the process parameters of producing.trehalose synthase by Arthrobacter nicotinovorus fermentation. [ Method] The influence of fermentation temperature, initial pH, rotational speed, inoculation amount and fermentation time on trehalose synthase enzyme activity producing by Arthrobacter nicotinovorus fermentation were studied through single factor experiment, and approximately determined the a-mount of the various factors. Then the rotational speed, the inoculation amount and the fermentation time were selected as three factors, the center combination of Box-Behnken design experiments was used to study the change of trehalose synthase enzyme activity in the fermentation as response value. The experiment dates were analyzed by the SAS statistical software to set up quadratic response surface regression model for obtaining the the optimal level of the important factor, determining the optimum conditions of fermentation. [ Result ] The response surface method results showed that the optimum condition of producing trehalose synthase by Arthrobacter nicotinovorus fermentation was as follow; the rotational speed 172.60 r/min, the inoculation amount 13.96% and the fermentation time 55.97 h, in the optima conditions, the trehalose synthase enzyme activity was 645.48 U/g. [Conclusion] The study can provide scientific basis for improving the enzyme activity of trehalose synthase produced by Arthrobacter nicotinovorus fermentation.关键词
海藻糖合成酶/海藻糖/食尼古丁节杆菌/发酵/响应面法Key words
Trehalose synthetase/ Trehalose/ Arthrobacter nicotinovorus/ Fermentation/ Response surface methodology分类
农业科技引用本文复制引用
张良,肖勇生,包珍,汤峰,刘媛洁,郭德斌..响应面法优化食尼古丁节杆菌发酵产海藻糖合成酶的工艺[J].安徽农业科学,2012,40(32):15873-15876,4.基金项目
农业科技成果转化资金项目(2010GB2C500236). （2010GB2C500236）
