安徽农业科学2012，Vol.40Issue(33)：16052-16053,16080,3.
红掌组培快繁技术优化研究
Study on Optimized Micropropagation of Anthurium andraeanurn
汪希强 1陶佩琳 1张旭东 1黄秀丽1
作者信息
- 1. 徐州生物工程职业技术学院,江苏徐州221006
- 折叠
摘要
Abstract
[Objective] To optimize micropropagation of Anthurium andraeanum. [ Method ] The effects of different basic medium and the combination of growth regulators on the adventitious bud inducement, proliferation and rootage were studied to find out the optimum formulation of Anthurium andraeanum. [ Result] The results showed that the best medium of callus inducement and adventitious bud differentiation was MS + 6-BA 1.0 mg/L + 2,4-D 0. 1 mg/L 4 NAA 0.1 mg/L. The rate of callus inducement and adventitious bud differentiation was 73. 3% and 90. 9% respectively. The suitable number and size were found on the proliferation medium MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L. The optimum rooting medium was 1/2MS + NAA 0.2 mg/L supplemented with active carbon 1.0 g/L. The adventitious shoots grew best with the survival rate 95.6% on the transplant medium which the ratio of perlite and peat soil is 1 to 1. [ Conclusion] The study will provide reference for large-scale production of Anthurium andraeanurn.关键词
红掌/组织培养/优化/最佳配方Key words
Anthurium andraeanurn/ Tissue culture/ Optimization/ Optimum formulation分类
农业科技引用本文复制引用
汪希强,陶佩琳,张旭东,黄秀丽..红掌组培快繁技术优化研究[J].安徽农业科学,2012,40(33):16052-16053,16080,3.
