安徽农业科学2012，Vol.40Issue(33)：16094-16095,2.
采用ISSR-PCR分子标记法鉴别黑果枸杞与雄性不育枸杞
Identification of Lycium ruthenicum Murr and Male Sterility of Lycium barbarum L. by Inter-Simple Sequence Repeat Markers
摘要
Abstract
[Objective] To identify Lycium ruthenicum and male sterility of Lycium barbarum by inter-simple sequence repeat markers. [ Method] Primers suitable for routine analysis were screened from 100 inter-simple sequence repeat primers, then, they were used in PCR and separated of 2 samples of L. Ruthenicum and male sterility of /- barbarum L. [ Results] Four of the 100 primers amplified polymorphic bands and suitable for the identification of L ruthenicum and male sterility oft. Barbarum. [Conclusion] Inter-simple sequence repeat markers provide a quick, reliable molecular marker for identification of L ruthenicum and male sterility of L barbarum.关键词
枸杞/ISSR/鉴别Key words
Lycium barbarum L. / ISSR/ Identification分类
农业科技引用本文复制引用
思彬彬,张靠稳,刘国迪..采用ISSR-PCR分子标记法鉴别黑果枸杞与雄性不育枸杞[J].安徽农业科学,2012,40(33):16094-16095,2.基金项目
北方民族大学资助项目(2010Y045). （2010Y045）
