安徽农业科学2012，Vol.40Issue(33)：16102-16106,5.
枳早期结瘤素样基因PtBCP1的克隆和分析
Clone and Analysis of A Phytocyanin-related Early Nodulin-like gene, PtBCP1 from Poncirus trifoliate
摘要
Abstract
[Objective] The aim was to clone and analyze the sequence of phytocyanin-related early nodulin-like gene (PtBCPl) from Poncirus trifoliata. [Method] The electronic cloning was done by using the C28 EST of Poncirus trifoliata subtractive library as seed sequences and the RT-PCR primers were designed by that. Then the PtBCPl cDNA and DNA of Poncirus trifoliate were cloned by PCR and the sequences were analyzed by bioinformatics methods. [Result] The PtBCPl gene included two exons and one intron, and was 140 times of expression in the seeding root of Poncirus trifoliata than that of in leaves. The gene encoded a polypeptide of 131 amino acids with a 14 kD molecular weight and 8.75 isoelectric point. The PtBCPl protein was a early nodulin-like protein, with a signal peptide in the N-terminal and a plastocyanin-lifce domain in the C-terrainal, and without the complete copper-binding sites. [ Conclusion ] It prepared the ground for the further research of PtBCPl gene biological function.关键词
枳/早期结瘤素样基因/克隆Key words
Poncirus trifoliata/ Early nodulin-like gene/ Clone分类
农业科技引用本文复制引用
姚利晓,马园园,李凤龙,许兰珍,雷天刚,彭爱红,何永睿,陈善春..枳早期结瘤素样基因PtBCP1的克隆和分析[J].安徽农业科学,2012,40(33):16102-16106,5.基金项目
长江学者和创新团队发展计划项目(PCSIRT) （PCSIRT）
柑桔产业技术体系(CARS-27)农业部公益性行业(农业)科研专项(201003067-02-4) （CARS-27）
重庆市自然科学基金计划项目(cstc2011jjA811). （cstc2011jjA811）
