安徽农业科学2012，Vol.40Issue(33)：16152-16154,3.
定安鹅IGF1基因克隆及生物信息学分析
Cloning and Bioinformatics Analysis of IGF1 Gene in Dingan Goose
摘要
Abstract
[Objective] The research aimed to study the structural characteristics of IGFl gene in Dingan Goose and the functional prediction and analysis of its encoding proteins. [Method] cDNA sequence of IGFl gene was cloned and analyzed using RT - PCR and bioinformatics technique. [Result] Through gene cloning and analysis, a cDNA sequence with the size of 558 bp was ontained, which included a 462 bp whole ORF and encoded 153 amino acid residuals. Blast analysis showed that IGFl gene in goose was very conservative in evolution. The signal peptide prediction analysis showed that the first 49 amino acid residuals in N - terminal of IGFl protein in Dingan Goose were signal pep-tide sequences. The subcellular localization analysis indicated that the mature protein of IGFl in Dingan Goose was located outside the cells. [ Conclusion] The research could lay the foundation for further study on the functions of IGFl gene in Dingan Goose.关键词
定安鹅/IGF1/肌肉发育/生物信息学Key words
Dingan Goose/ IGFl/ Muscle development j Bioinformatics分类
农业科技引用本文复制引用
叶保国,张小辉,徐铁山,谢亮..定安鹅IGF1基因克隆及生物信息学分析[J].安徽农业科学,2012,40(33):16152-16154,3.基金项目
国家水禽产业技术体系海口综合试验站项目(CARS-43-42). （CARS-43-42）
