安徽农业科学2012，Vol.40Issue(33)：16392-16394,16500,4.
超声波辅助双水相提取虫草多糖的工艺研究
Extraction Conditions of Polysaccharide from Cultured Cordyceps militaris by Ultrasonic Assisted Aqueous Two Phase System
摘要
Abstract
[Objective] To optimize the extraction process of polysaccharide from cultured Cordyceps militaris by ultrasonic assisted aqueous two phase system. [Method] Orthogonal design was used to optimize extracting parameter and the content of polysaccharide was determined with phenol-sulfuric acid. [Result] The optimum extraction conditions of polysaccharide were: fruiting body of powder (100 mesh) , solid-liquid ratio of 0.03 g/ml, extraction time of 3 h, temperature of 60 °C , ultrasonic time of 30 min at which the highest extraction rate of polysac-charides was 30. 1 %. [ Conclusion] The optimum extraction process of polysaccharide was obtained, and provided the basis for its further development.关键词
双水相体系/虫草多糖/提取工艺Key words
Aqueous two phase system/ Cordyceps polysaccharide/ Extraction process分类
农业科技引用本文复制引用
孙诗清,王玉洁,朱长俊,徐薇,刘晓侠..超声波辅助双水相提取虫草多糖的工艺研究[J].安徽农业科学,2012,40(33):16392-16394,16500,4.基金项目
嘉兴市科技局项目(2011AY1061) （2011AY1061）
嘉兴学院优秀青年教师资助项目(70611007). （70611007）
