安徽农业科学2012，Vol.40Issue(35)：17007-17010,17028,5.
毛竹萌发种子全长cDNA文库的构建
Construction of Full-length cDNA Library for Germinating Seeds of Phyllostachys heterocycla
摘要
Abstract
[Objective]This study aimed to construct the full-length cDNA library for germinating seeds of Phyllostachys heterocycla. [Method]Germinating seeds of P. heterocycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a capacity of 6. 5 × 106 recombinant clones in total,and a low proportion of clones without inserted frag-menst; the size of inserted fragments ranges between 0.3 -S.0 kb.with strict classification and ideal consistency. Furthermore,the proportion of clones with long inserted fragments (1.0 -5.0 kb) was as high as 30% .achieving the standard for high-quality full-length cDNA library. [Conclusion] The full-length cDNA library for germinating seeds of P. heterocycla was successfully constructed .which laid important foundation for the functional genomics research of bamboo plants.关键词
毛竹/全长cDNA文库/萌发种子/Oligo-capping法Key words
Phyllostachys helerocycla/ Full-length cDNA library/ Germinating seeds/ Oligo-capping method分类
农业科技引用本文复制引用
胡陶,姚娜,杨学文,李雪平,牟少华,马艳军,高志民,彭镇华,李潞滨..毛竹萌发种子全长cDNA文库的构建[J].安徽农业科学,2012,40(35):17007-17010,17028,5.基金项目
国际竹藤中心基本科研业务费专项(10618-10) （10618-10）
林业公益性行业科研专项(200704001) （200704001）
国际竹藤中心基本科研业务费专项(12618-7). （12618-7）
