安徽农业科学2012，Vol.40Issue(35)：17026-17028,3.
部分桑树品种花粉亚显微形态观察及比较
Observation and Comparison of Pollen Submicroscopic Morphology of Some Mulberry (Morus alba Linn.) Cultivars
摘要
Abstract
[Objective] This study aimed to investigate the submicroscopic structure and morphological characteristics of mulberry pollen. [Method] Mulberry pollen of seven cultivars were processed by glutaraldehyde fixing,alcohol gradient dehydration,freeze - drying and metal plating to observe the size and surface ornamentation under the scanning electron microscope (SEM). [Result] The pollen surface characteristics and ornamentation were clearly visible,indicating this method is suitable for SEM observation of mulberry pollen; experimental mulberry pollen was nearly spherical,with two germ pores and spike on pore membrane; polar axis was 15.99-22.63 μm,and equatorial axis was 14.98 -20.78 μm; the pollen volume of 'Yu No. 2' was the maximum,while that of 'Jinxuan No. 7' was the minimum; the surface of mulberry pollen showed smooth and tumor - like protuberances ornamentation, covered with different density of particles. [Conclusion]Glutaraldehyde fixative - alcohol gradient dehydration is an ideal method for morphological characteristics of mulberry pollen. This study may provide useful information for palynology identification of Morus plants or even species -level systematic classification.关键词
桑树/花粉/亚显微形态Key words
Mulberry/ Pollen/ Submicroscopic morphology分类
农业科技引用本文复制引用
胡德昌,张萍,王艳杰,左进城,王照红,陈传杰..部分桑树品种花粉亚显微形态观察及比较[J].安徽农业科学,2012,40(35):17026-17028,3.基金项目
国家自然科学青年基金(31000308) （31000308）
山东省优秀中青年科学家科研奖励基金(BS2010NY006). （BS2010NY006）
