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六堡茶茶褐素的提取工艺及其抗氧化活性研究

何英姿 逯钊琦 陈艳芳 刘艳妮 黄小燕 郑维志

安徽农业科学2012,Vol.40Issue(35):17301-17303,17310,4.
安徽农业科学2012,Vol.40Issue(35):17301-17303,17310,4.

六堡茶茶褐素的提取工艺及其抗氧化活性研究

Extraction Technology of Theabrownine from Liubao Tea and Its Antioxidant Activity Research

何英姿 1逯钊琦 1陈艳芳 1刘艳妮 1黄小燕 1郑维志1

作者信息

  • 1. 广西师范学院化学与生命科学学院,广西南宁530001
  • 折叠

摘要

Abstract

[Objective]To provide theoretical reference for theabrownine extraction from Liubao tea, biological activity determination, development and utilization. [Method] Orthogonal test was adopted to investigate effects of solid-liquid ratio, extracting temperature, time, times on yield of Liubao tea theabrownine, the technique was optimized, and the antioxidant activity of theabrownine was analyzed. [Result] The results showed that effects of extraction times and temperature on theabrownine yield reached significant level, while other two factors were not significant. The optimum extraction conditions were determined as follows: solid-liquid ratio 1: 30, extraction temperature 100 ℃ , extraction time 80 min, extraction 4 times. Under this condition, the yield could be up to 22. 92%. Results of antioxidant activity research showed that the most effectively scavenging activity of hydroxyl radical could be approached to 74% , stronger than Vitamin C' s; And the scavenging activity of Nitroso could be approached to 73% , and there was little difference compared with Vitamin C' s; Although the scavenging activity of superoxide radical was no better than Vitamin C' s, it also could be approached to 60%. [Conclusion]liubao tea has abundant theabrownine, and theabrownine has strong antioxidant activity, which will lay a solid foundation for deeply development of Liubao tea.

关键词

六堡茶/茶褐素/提取/抗氧化活性

Key words

Liubao tea/ Theabrownine/ Extraction/ Antioxidant activity

分类

农业科技

引用本文复制引用

何英姿,逯钊琦,陈艳芳,刘艳妮,黄小燕,郑维志..六堡茶茶褐素的提取工艺及其抗氧化活性研究[J].安徽农业科学,2012,40(35):17301-17303,17310,4.

基金项目

广西教育厅科研基金面上项目(201012MS138). (201012MS138)

安徽农业科学

0517-6611

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