安徽医科大学学报2012，Vol.47Issue(12)：1393-1396,4.
生活方式对高脂肥胖大鼠APPL1和GLUT4的影响
The effects of life style on APPL1 and glucose transporter 4 in high fat induced obese rats
摘要
Abstract
Objective To investigate the effects of diet control and exercise on APPL1, glucose transporter 4 ( GLUT4 ) and their correlation in high fat diet induced obese rats. Methods 70 SD rats of 4 weeks, age were randomly divided into five groups: normal control group ( NC group ), high-fat diet group( HF group ), diet control group ( DC group ),exercise control group( EC group )and diet and exercise control group( DE group ). The animals of NC group were fed with normal chow and the others with high-fat diet. After 12 weeks, the rats of DC group were given diet control for 6 weeks, at the same time, EC group swam everyday, DE group had to do both two interventions. Then the blood of rats in fasting was obtained to measure metabolic indexs, triglyceride lipid and expression of AP-PL1, GLUT4 were measured in skeletal muscle tissues. Results Body weight, triglyceride( TG ), total cholesterol ( TCH ), fasting blood glucose( FBG ), fasting insulin( FINS ) and the TG level in skeletal muscle tissue of HF group were significantly higher than those of NC group( P < 0. 05 )expression of APPL1, GLUT4 of HF group were significantly lower. Diet, exercise intervention could reduce body weight( P < 0. 05 ), decrease TG, TCH( P < 0. 05 ), increase insulin sensitivity index( P < 0. 05 ), decrease glycerin level in skeletal muscle tissue( P < 0. 05 ), and enhance the expression of APPL1, GLUT4( P < 0. 05 ). Conclusion High fat diet could induce the model of insulin resistance. Diet control and/or exercise can reduce lipid levels of high-fat diet, improve insulin resistance and up-regulate expressions of APPL1, GLUT4 in skeletal muscle tissue.关键词
APPL1/GLUT4/肥胖/运动/饮食/大鼠Key words
APPL1/ GLUT4/ obesity/ exercise/ diet/ rats分类
医药卫生引用本文复制引用
童俊露,王佑民..生活方式对高脂肥胖大鼠APPL1和GLUT4的影响[J].安徽医科大学学报,2012,47(12):1393-1396,4.基金项目
安徽省科技攻关项目(编号:1206C0805034) （编号:1206C0805034）
安徽省高等学校省级自然科学研究重点项目(编号:KJ2011A161) （编号:KJ2011A161）
