安徽医科大学学报2012，Vol.47Issue(12)：1397-1400,4.
低氧诱导对胃癌细胞发生上皮间质转化的影响
Effects of hypoxia-induced epithelial-mesenchymal transition in gastric cancer cells
史冠男 1戴夫 1夏阳 2刘尚全 3袁媛 3彭琼 1甘惠中1
作者信息
- 1. 安徽医科大学第三附属医院消化内科,合肥,230061
- 2. 安徽医科大学第三附属医院肿瘤内科,合肥,230061
- 3. 合肥市滨湖医院中心实验室,合肥,230601
- 折叠
摘要
Abstract
Objective To investigate whether hypoxia can induce epithelial-mesenchymal transition in gastric cancer cells SGC-7901. Methods After SGC-7901 cells were cultured under hypoxic conditions for different hours ( 0, 6, 12, 24,48 h ), cell morphological changes were observed. The expression of HIF-2α, E-cadherin, N-cadherin, Vimentin were detected by Western blot. The scratch wound assay was used to assess the invasion and migration levels of SGC-7901 cells. Results After exposure to hypoxia for 48 h, SGC-7901 cells changed gradually into the shape of fusiform and generated pseudopodium. The expression of HIF-2α, N-cadherin, Vimentin were up-regulated ( P < 0. 05 )and E-cadherin was significantly down-regulated( P < 0. 05 ). The invasion and migration levels of hypoxi-a-treated cells were increased as compared with those of control group. Conclusion Hypoxia can increase the likelihood of the epithelial-mesenchymal transition in gastric cancer cells and improve their invasion and migration levels.关键词
低氧/上皮间质转化/胃癌Key words
hypoxia/ epithelial-mesenchymal transition/ gastric cancer分类
医药卫生引用本文复制引用
史冠男,戴夫,夏阳,刘尚全,袁媛,彭琼,甘惠中..低氧诱导对胃癌细胞发生上皮间质转化的影响[J].安徽医科大学学报,2012,47(12):1397-1400,4.
