安徽中医学院学报2012，Vol.31Issue(6)：51-55,5.DOI:10.3969/j.issn.1000-2219.2012.06.020
重楼总皂苷对人胃癌MNK-45和MGC80-3细胞增殖的影响
Effects of Rhizoma Paridis Total Saponins on Growth of Gastric Carcinoma Cell Lines MNK-45 and MGC80-3
摘要
Abstract
To observe the effects of Rhizoma Paridis total saponins (RPTS) on the growth of human gastric carcinoma cell lines MNK-45 and MGC80-3 . Methods MNK-45 and MGC80-3 cells were treated with 5 , 10 , 20 , 40 , and 80 μg/ml RPTS for 24 , 48, and 72 h, and then methyl thiazolyl tetrazolium (MTT ) assay was used to measure the growth inhibition rates of the two cells . MNK-45 and MGC80-3 cells were treated with 10 jug/ml RPTS for 24 h , and then acridine orange/ethidium (AO/EB) staining was used to observe the morphologi -cal changes of the two cells . MNK-45 and MGC80-3 cells were treated with 10 and 20 jug/ml RPTS , and then flow cytometry was used to determine the changes in cell cycles of the two cells . Results RPTS (5-80 jug/ml) had significant inhibitory effects on the growth of MNK-45 and MGC80-3 cells (P<0 .05 or P<0 .01), and the higher the dosage was , the longer and stronger the inhibitory effect was (P<0 .01) . RPTS had significantly stronger inhibitory effects on MNN-45 cells than on MGC80-3 cells when applied at the same concentration for the same peri -od (P< 0.01). Both cells showed apoptotic features under a fluorescence microscope after being treated with RPTS. Treated with RPTS, MNK-45 cells were arrested in Go /Gi phase, and MGC80-3 cells in S phase. Conclusion RPTS can inhibit cell growth , induce apoptosis , and affect cell cycle in human gastric carcinoma cell lines MNK-45 and MGC80-3 , but the effects on cell growth and cell cycle were different in them .关键词
重楼总皂苷/人胃癌细胞株/增殖抑制作用/细胞周期/细胞凋亡Key words
Rhizoma Paridis total saponins / human gastric carcinoma cell line / inhibitory effect on growth/ cell cycle / cell apoptosis分类
医药卫生引用本文复制引用
保永亮,龚晓燕,方海雁,黄金玲,王桐生,刘先华,王训翠..重楼总皂苷对人胃癌MNK-45和MGC80-3细胞增殖的影响[J].安徽中医学院学报,2012,31(6):51-55,5.基金项目
国家自然科学基金项目(81173171) （81173171）
