北京中医药大学学报2012，Vol.35Issue(11)：757-760,后插3,5.
四君子汤联合快速康复外科干预肝脏缺血再灌注损伤的实验研究
Intervention of hepatic ischemia-reperfusion injury by Sijunzi Tang combining fast-track surgery
摘要
Abstract
Objective To investigate the intervention effect of Sijunzi Tang combining fast-track surgery (FTS) on hepatic ischemia-reperfusion injury. Methods Health SD rats (n =60) were randomly divided into sham-operation group, non-FTS group and FTS group, and FTS group was divided further into routine group and Chinese medicinal group (FTS + CM group, each n = 15). The model of obstructive jaundice was established by common bile duct ligation in all rats, and FTS groups and non-FTS group were given the operation of hepatic ischemia-reperfusion. FTS + CM group was additionally given Chinese medicinals ( Sijunzi Tang). After 24 hours, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) and superoxide dismutase (SOD) were detected. The morphological changes of liver were observed after HE staining. The in situ apoptosis of liver cells was detected by using TUNEL and apoptosis index ( AI) was calculated. Results The comparison in the levels of ALT and AST showed that there was no significant difference between sham-operation group and FTS groups (P > 0. 05 ) , there was a difference between sham-operation group and non-FTS group (P < 0. 05) , there was a difference between non-FTS group and FTS groups (P < 0. 05) , and there was a difference between routine group and FTS + CM group ( P < 0. 05 ). The comparison in level of MDA showed there was no difference among all groups (P >0. 05). The comparison in level of SOD showed that there was no difference between sham-operation group and routine group (P >0. 05) , and there were differences in the comparison among other groups ( P < 0. 05 ). The morphological observation showed that the injury was milder in FTS groups than that in non-FTS group. AI was significantly lower in sham-operation group than that in other groups (P<0.01) , and higher in non-FTS group than that in FTS groups ( P < 0. 05 ). Conclusion The intervention of FTS ameliorates the apoptosis of liver cells, reduce the injury degree of liver cells and liver function induced by ischemia-reperfusion in jaundice rats.关键词
快速康复外科/肝脏缺血再灌注损伤/天冬氨酸转氨酶/谷氨酸转氨酶/丙二醛/超过氧化物歧化酶/细胞凋亡/大鼠Key words
fast-track surgery/ hepatic ischemia-reperfusion injury/ aspartate aminotransferase/malondialdehyde/ superoxide dismutase/ apoptosis/ rats分类
医药卫生引用本文复制引用
傅永清,华晨,陈炳荣,傅天啸,张军,周剑..四君子汤联合快速康复外科干预肝脏缺血再灌注损伤的实验研究[J].北京中医药大学学报,2012,35(11):757-760,后插3,5.基金项目
浙江省教育厅基金项目(No.Y201018053) （No.Y201018053）
