北京中医药大学学报2012，Vol.35Issue(11)：781-785,5.
寿胎丸调节原因不明复发性自然流产患者免疫失衡机制
Reversed effect of Shoutai Wan on immunologic dissonance through regulating mononuclear granulocytes in patients with unknown recurrent spontaneous abortion (type of kidney deficiency)
摘要
Abstract
Objective To discuss the mechanism of Shoutai Wan in fetus protection through detecting the expressions of T-bet and GAT A3 mRNA,and level changes of interleukin-2 (IL-2 ) , interferon-γ(IFN-γ) , IL-4 and IL-10 in the patients with unknown recurrent spontaneous abortion ( URSA) before and after treatment with Shoutai Wan. Methods The patients ( n - 50) were randomly divided into Chinese medicinal group (n - 30) treated with Shoutai Wan, and control group (n = 20 ) , and a normal group ( n =20) was set up including women with normal early pregnancy. Two samples of peripheral vein blood were collected from the patients of three groups respectively, one was centrifugalized out plasma for detecting the levels of interleukin-2 (IL-2) , interferon-7 (IFN-7) , IL-4 and IL-10 by using ELISA, and other one was centrifugalized out peripheral blood mononuclear cells ( PBMCs ) with gradient centrifugation according to the density. The expressions of T-bet mRNA and GATA-3 mRNA in PBMCs were detected by using RT-PCR. Results Before the treatment, the levels of IL-2 and IFN-γ and expression of T-bet mRNA were higher, and levels of IL-4 and IL-10 and expression of GATA-3 mRNA were lower in the patients than those in normal group (P<0.05). The expression of T-bet mRNA was correlated to levels of IL-2 and IFN-7 and negatively correlated to levels of IL-4 and IL-10. The expression of GATA-3 mRNA was correlated to levels of IL-4 and IL-10 and negatively correlated to levels of IL-2 and IFN-γ. After the treatment, the levels of IL-4 and IL-10 increased, expression of GATA-3 mRNA significantly increased, and levels of IL-2 and IFN-7 and expression of T-bet mRNA decreased in the patients. The abortion rate was lower in Chinese medicinal group than that in control group with significant difference (P<0.05). Conclusion Shoutai Wan can regulate the ratio of T-bet to GATA-3 through up-regulating the expression of GATA-3 mRNA, down-regulating the expression of T-bet mRNA, and then up-regulates the levels of IL-4 and IL-10 , down-regulates the levels of IL-2 and IFN-7, reverses the imbalance of Thl/Th2 cells and maintains normal pregnancy.关键词
原因不明复发性自然流产/寿胎丸/Th1/Th2/T-bet/GATA-3Key words
unknown recurrent spontaneous abortion/ Shoutai Wan/ Thl/Th2/ T-bet/GATA-3分类
医药卫生引用本文复制引用
王力,闻姬,韩彩艳..寿胎丸调节原因不明复发性自然流产患者免疫失衡机制[J].北京中医药大学学报,2012,35(11):781-785,5.基金项目
山东省中医药科技发展计划项目(No.2011-126) （No.2011-126）
