草业学报2012，Vol.21Issue(6)：57-63,7.
铅梯度胁迫对多年生黑麦草幼苗生理生化特性影响
Influence of lead gradient stress on the physiological and biochemical characteristics of perennial ryegrass (Lolium perenne) seedlings
摘要
Abstract
The effects of lead concentrations (500, 1 000, 1 500 mg/L) on the antioxidant enzymes (SOD, POD), cell membrane (MDA, the relative conductivity) and osmoregulation substances (soluble sugar and proline) in the leaves of perennial ryegrass {Lolium perenne) were studied after different stress durations (7, 14, 21, and 28 d) in a pot experiment. SOD activity increased initially and then decreased with the increase in lead concentration. At lower concentrations (500, 1 000 mg/L) and shorter durations (7, 14 d) , SOD activity significantly increased but then declined rapidly. POD activity decreased significantly with increases in stress duration and lead concentrations. POD activity was minimum (45. 9 U/g) at the 28th day and 1 500 mg/L of lead. With increases in stress duration and lead concentrations, MDA content and the relative conductivity generally increased. MDA content was positively linearly correlated with lead concentration (R2>0. 96). Soluble sugar content showed complex change trends, and tended to reduce at the higher concentrations and longer durations. Proline content significantly increased at the lower lead concentrations. If lead concentration exceeded the limit of perennial ryegrass tolerance, proline content would decline. It indicated that perennial ryegrass can be tolerant to lead stress but there is progressive damage under the stress.关键词
铅胁迫/黑麦草/抗氧化酶/细胞膜/渗透调节物质Key words
lead stress/ perennial ryegrass (Lolium perenne) / antioxidant enzyme/ cell membrane/ osmoregulation substance分类
农业科技引用本文复制引用
刘慧芹,韩巨才,刘慧平,康欣然..铅梯度胁迫对多年生黑麦草幼苗生理生化特性影响[J].草业学报,2012,21(6):57-63,7.基金项目
山西省留学基金项目(2009043,2011-52)和山西省科技攻关项目(20100311038,20110311013-1)资助. （2009043,2011-52）
