草业学报2012，Vol.21Issue(6)：166-174,9.
苜蓿乙烯应答因子基因的表达特性和生物信息学分析
Bioinformatics and expression analyses of ethylene response factor genes in Medicago
摘要
Abstract
Ethylene response factors are plant-special transcription factors involved in plant growth and development. The Arabidopsis AP2 domain was used as a probe to search the NCBI Medicago protein database and to analyze the phyletic evolution, secondary structure, hydrophobicity, signal peptide, subcellular localization of Medicago ethylene response factors using bioinformatics software. Primers were designed based on the sequences obtained to analyze the expression patterns of MsERF genes (MsERFl, MsERF2, MsERF6, MsERFl, MsERF9). The MsERF genes had high expression levels in leaves and showed significant differences of expression in different tissues. The expression of MsERF2 was not induced by NaCl and PEG-6000, but was promoted by A12(S()4)3. The expression of other genes was induced by these three treatments, but the expression patterns were different. ABA reduced the expression of these genes except for MsERF2. IAA had no effect on the expression of these genes except MsERFS. GA3 , MeJA, and Eth reduced the expression of all five genes. This research established a solid foundation to further study the function of these genes and provided evidence to study the cross-talk in different signal pathways.关键词
紫花苜蓿/乙烯应答因子/生物信息学分析/半定量RT-PCR/表达分析Key words
Medicago sativa / ethylene response factor/ bioinformatics analysis/ semi-quantitative RT ?PCR/expression analysis分类
农业科技引用本文复制引用
陈婷婷,杨青川,张新全,康俊梅,丁旺,张铁军..苜蓿乙烯应答因子基因的表达特性和生物信息学分析[J].草业学报,2012,21(6):166-174,9.基金项目
国家现代牧草产业技术体系(CARS-35)和国家自然科学基金(30871819)资助. （CARS-35）
