成都大学学报：自然科学版2012，Vol.31Issue(4)：305-308,4.
离心-冷冻法制备梯度孔结构的真皮支架
Dermis Scaffold of Gradient Pore Structure Fabricated by Centrifugal-freeze Method
摘要
Abstract
There are many factors controlling the microstructure and properties of collagen-based dermis scaffold, such as other main materials, the pore diameter and porosity as well as the cross-linking density. Dermis scaffold of gradient pore structure was fabricated by combination of centrifugal-freeze method and freeze-drying method. The pore diameter and porosity of the scaffold were investigated by scanning electron microscope. Biocompatibility and cell adhesion properties of the scaffold were tested by experiment of cell culture growth to characterize the influence of centrifugal force. The results show that the centrifugal force has important influence on the microstructure of dermis scaffold with different degree of densification from the bottom up on the three layers and the pore diameters are different from each other, like the upper is 52 ± 27.8 μm, the middle is 57 ± 8.7 μm and the lower is 49 ± 40.4 μm. Moreover, the results of cell adhe- sion experiment show that the lower layer is more suitable for the adhesion and growth of cell among the three different structures.关键词
真皮支架/离心力/生物相容性Key words
dermis scaffold/centrifugal force/biocompatibility分类
医药卫生引用本文复制引用
王明波,王俊,谭荣伟,余振定..离心-冷冻法制备梯度孔结构的真皮支架[J].成都大学学报：自然科学版,2012,31(4):305-308,4.基金项目
广东省教育部产学研结合项目（201013090400324）,深圳市生物、互联网、新能源产业发展专项基金（CSB7010C5260047A）资助项目. （）
