重庆医学2012，Vol.41Issue(35)：3734-3736,封4,4.DOI:10.3969/j.issn.1671-8348.2012.35.019
当归多糖对辐射损伤小鼠造血系统保护作用的研究
Studys of angelica polysaccharides on the protection of ionizing radiation in injured mice
摘要
Abstract
Objective To study the effects of angelica polysaccharide(APS) on the damage of bone marrow by ionizing radiation in injured mice,so as to illuminate the protective effects of APS against radioactive haematogenesis injury. Methods Using the line ar accelerator irradiate C57BL/6 mice one time with 4. 0 Gy X ray to establish the animal radiation injured model. Then these mice were killed and extracted BMNC after 7 days injection. Counting the number of the blood cells and BMNCs and observing the pa thology change of bone marrow. The cell cycle and apoptosis rate were detected by flow cytometry and the expression of p53 was detected by immunocytochemistry. Results Compared with normal group,the percentage of Go/Gj phase,apoptosis rate and the ex pression of p53 increased significantly,while the number of the blood cells and BMNCs,the number of cells in bone marrow obvi ously decreased in NS group;both in the 2 mg/kg APS and 8 mg/kg APS group,the percentage of Go/Gi phase and apoptosis rate decreased, while t he number of the blood cells and BMNCs, the number of cells in bone marrow increased. Conclusion APS can improve the fuction of bone marrow hematopoietic system and have protection in ionizing radiation.关键词
辐射损伤/造血系统/细胞周期/细胞凋亡/当归多糖Key words
radiation injuries/hematopoietic system/cell cycles/apoptosis/angelica polysaccharide引用本文复制引用
何晓莉,张雁,吴宏,姜蓉..当归多糖对辐射损伤小鼠造血系统保护作用的研究[J].重庆医学,2012,41(35):3734-3736,封4,4.基金项目
重庆市渝中区科技计划项目(20110321). （20110321）
