重庆医学2012，Vol.41Issue(36)：3809-3811,3.DOI:10.3969/j.issn.1671-8348.2012.36.004
大肠癌中miRNA-20a的表达与TNM分期和淋巴结转移相关性研究
A correlation research of the expression of miRNA-20a and TNM staging in colorectal cancer and the lymph node metastasis
马群英 1江美玲 1马凤丽 1李钊 1许岸高2
作者信息
- 1. 南方医科大学附属南方医院胃肠科,广州,510515
- 2. 惠州市第一人民医院/惠州市医学研究所,516001
- 折叠
摘要
Abstract
Objective To investigate the expression of miRNA 20a in colorectal cancer and its relation with clinical pathology features. Methods Total RNA were extracted from 52 paired colorectal cancer(CRC) tissues and para cancer normal tissues which were excised surgically. miRNA 20a was detected by real time PCR with TaqMan probe. Then,the clinical significance of miRNA 20a was analyzed. Results The expression level of miRNA 20a in CRC tissues was 2. 05(0. 046-10. 274) ,which was significantly higher than para cancer normal tissues(Z=-3. 597,P = 0. 000) ; and the expression of miRNA 20a was correlated with TNM stag ing(T=3. 223,P = 0. 001) ,tissues with higher TNM stages expressed higher miRNA 20a. miRNA 20a levels were also related to lymph node invasion(Z=-2. 141, P = 0. 037), tissues with positive node invasion expressed higher miRNA 20a. No significant correlation was found between miRNA 20a and gender, age, tumor site and tissue differentiation. Conclusion The upregulation of miRNA 20a might play a role in tumorigenesis and metastasis of colorectal cancer.关键词
肠肿瘤/miRNA-20a/TNM分期Key words
intestinal neoplasms/ miRNA 20a/ TNM staging引用本文复制引用
马群英,江美玲,马凤丽,李钊,许岸高..大肠癌中miRNA-20a的表达与TNM分期和淋巴结转移相关性研究[J].重庆医学,2012,41(36):3809-3811,3.
