重庆医学2012，Vol.41Issue(36)：3854-3856,3.DOI:10.3969/j.issn.1671-8348.2012.36.022
地塞米松对哮喘小鼠气道血管生成及血管内皮生长因子表达的影响
Effects of dexamethsone on airway angiogenesis and transforming vascular endothelial growth factor in chronic asthma mice
姚红卫 1吕丽丽2
作者信息
- 1. 江苏省常州市第四人民医院呼吸内科,213001
- 2. 徐州医学院附属医院老年科,江苏徐州,221008
- 折叠
摘要
Abstract
Objective To investigate the effects of dexamethsone on airway angiogenesis and transforming vascular endothelial growth factor in chronic asthma mice. Methods The asthmatic model was established by sensitization and challenge with OVA. The mice were randomly divided into three groups ,control group,asthma group and dexamethsone treatment group. After the last chal lenge,eosinophile(EOS) counts and active VEGF level in bronchoalveolar lavage fluid(BALF) were calculated; the pathologic chan ges of bronchi and the lung tissue were evaluated; Anti Factor YUAg antibody was used to detect vessels ,The number of vessels in lamina propria and submucosa of tracheae was counted microscopically; Immuno his to chemistry were used to measure the level of VEGF in the lung tissues of asthmatic mice;the airway wall thickness(WAt/Pbm) and vascular counts were measured by using im age analysis system. Results EOS increased significantly in asthma group compared to that in Dexamethsone group,while between dexamethsone group and control group there was no significantly difference. After repeatedly OVA exposure,the density of vessels and airway wall thickness(WAt/Pbm) and the number of vascular in lung tissues were increased significantly compared with those of control group,which were also positively related with the concentrations of VEGF in lung tissue(P<0. 05). Conclusion Dexam ethsone can relieve the allergic inflammation of airway, and ease off airway angiogenesis in asthmatic mice. VEGF was over ex pressed in the asthmatic mice,and may play an important role in airway angiogenesis.关键词
血管内皮生长因子A/地塞米松/气道血管生成Key words
asthma/ vascular endothelial growth factor A/ dexamethsone/ airway angiogenesis引用本文复制引用
姚红卫,吕丽丽..地塞米松对哮喘小鼠气道血管生成及血管内皮生长因子表达的影响[J].重庆医学,2012,41(36):3854-3856,3.
