大豆科学2012，Vol.31Issue(6)：865-868,4.
大豆整体子叶节再生体系的建立及应用于农杆菌介导遗传转化初探
Cluster Bud Induction of Soybean Whole Cotyledonary Node and Application in Agrobacterium-mediated Genetic Transformation
摘要
Abstract
The effects of nine concentration combinations of 6-BA,IBA and KT designed by orthogonal designL9( 34 ) ]were evaluated on induction of soybean [ Glycine max(L.) Merrill] multi-shoot from whole cotyledonary nodes. Four soybean genotypes, including Zhouhuang 20, Zhoudou 02005 , Zhoudou 11 and Zhoudou 18, were used as plant materials, while MSB was used as basic medium. The results showed that nine concentration combinations had different effects on induction of multi-shoot for soybean whole cotyledonary nodes. The regeneration system was further used to Agrobacterium-mediated genetic transformation. For nine concentration combinations,MSB +3.0 mg·L-1'6-BA +0.1 mg·L-1 IBA +0.5 mg·L-1 KT for Zhoudou 02005 and MSB + 3.5 mg o L-1 6-BA +0.2 mg· L-1 IBA + 0.2 mg o L-1 KT for Zhouhuang 20, Zhoudou 11 and Zhoudou] 8 were opti mum to induce multi-shoot than other combinations. For four genotypes, the regeneration efficiency of multi-shoot for Zhouhuang 20 was the highest. The concentration of 0.8 mgoL-1 for IB A and 0.8-1.0mgoL-1 for NAA was much more suitable for soybean rooting,and rooting efficiency of Zhonghuang 20 was the highest. Agrobacterium-mediated transformation of Zhong-huang 20 was performed using whole cotyledonary nodes as explants,it showed that reporter gene( GUS)had been transformed into soybean genome by PCR analysis and GUS staining with 7.8% transformation efficiency, and the period of obtaining trans-genic plant was 35-42 d.关键词
大豆/整体子叶节/再生/农杆菌介导/遗传转化Key words
Soybean/Whole cotyledonary node/Regeneration/Agrobacterium-mediated/Genetic transformation分类
农业科技引用本文复制引用
张福丽,舒文涛,张怡,高丹,张丽,陆亚杰,罗祥,李成伟..大豆整体子叶节再生体系的建立及应用于农杆菌介导遗传转化初探[J].大豆科学,2012,31(6):865-868,4.基金项目
转基因生物新品种培育重大专项子任务(2008ZX08010-004) （2008ZX08010-004）
河南省教育厅自然科学项目(2012B210028,2011B180060) （2012B210028,2011B180060）
周口师范学院博士科研启动基金 （）
周口师范学院实验室开放项目基金. （）
