大豆科学2012，Vol.31Issue(6)：878-881,886,5.
拟南芥AtGPAT9基因cDNA的克隆及其转化大豆的研究
Cloning of AtGPAT9 cDNA from Arabidopsis and Its Transformation of Soybean
摘要
Abstract
Glycerol-3-phosphate acyltransferases(GPATs)play crucial role in the biosynthesis of triacylglycerols(TAGs) which are major form of the storage oils accumulated in developing seeds,and the enzymes catalyze the acylation of glycerol-3-phosphate at the sn-1 position and result in the formation of lysophosphatidic acid,which is the initial step of TAG biosynthesis. The endoplasmic reticulum-expressed AtGPAT9, a member of the GPAT family in Arabidopsis thaliana, was predicted to play an important role in the reaction. In the present study,the complete coding region cDNA of AtGPAT9) was cloned by using reverse transcriptase-mediated PCR,and the gene' s plant expression vector was constructed. Then soybean transformation was performed by the Agrobacterium tumefaciens-mediated cotyledon node method, and the regenerated plants resistant to selective a-gent phosphinothricin/PPT were obtained.关键词
甘油-3-磷酸酰基转移酶/基因克隆/大豆转化Key words
Glycerol-3-phosphate acyltransferases ( GPATs ) /Gene cloning/ Transformation of soybean分类
农业科技引用本文复制引用
吴兴,赵欢欢,张锋,李宏伟,王茅雁..拟南芥AtGPAT9基因cDNA的克隆及其转化大豆的研究[J].大豆科学,2012,31(6):878-881,886,5.基金项目
国家转基因生物新品种培育科技重大专项(2009ZX080042009B). （2009ZX080042009B）
