大豆科学2012，Vol.31Issue(6)：887-893,7.
不同生育时期大豆异黄酮合成相关酶基因表达的分析
Analysis of Gene Expression Underlying Soybean Isoflavone Synthesis Relative Enzymes at Different Growth Stages
摘要
Abstract
Real-time PCR was used to measure the relative expression quantity of seven isoflavone synthetic enzyme related genes(PAL,C4H ,ACL,CHS ,CHI ,IFS and F3H) in high isoflavone cv. Zhongdou27 , low isoflavone cv. Jiunong20,as well as their derived 130 F5;II RILs. High performance liquid chromatography ( HPLC) was adopted to survey isoflavone content in pod at different growth stages, at the same time, correlations between total/individual isoflavone content and the expression of partial genes in F5;11RILs were performed using SAS 8. 2. The relative expression quantity of(PAL,CHI,IFS and FiH was generally increased in reproductive stage than vegetative stage. Relative expression quantity of PAL,C4H ,CHS and IFS gene in leaf appeared a peak value at Rl stage,and the expression quantity of most selected genes appeared significant difference in pod between two variety was at R6 stage. At R6 stage,the relative expression quantity of PAL gene manifested positive correlation with genistein(GT)and total isoflavone content(TI) ;CHS exhibited positive relevance with daidzein(DZ) ,GT and TI but negative with glycitein ( GC ) ; IFS displayed positive correlation with DZ; F3H showed negative correlation with individual and total isoflavone contents. Results will faciliate the understanding of genetic mechanism of the variation underlying isoflavone content.关键词
大豆异黄酮/异黄酮合成相关酶基因/Real-time PCR/相对表达量Key words
Soybean isoflavone/Isoflavone synthesis relative enzyme genes/Real-time PCR/Relative expression quantity分类
农业科技引用本文复制引用
王艳,武林,孙梦阳,韩英鹏,赵雪,滕卫丽,李文滨..不同生育时期大豆异黄酮合成相关酶基因表达的分析[J].大豆科学,2012,31(6):887-893,7.基金项目
农业部大豆产业技术体系资助项目(CARS-04-PS04) （CARS-04-PS04）
国家"十二五"科技支撑计划课题(2011BAD35B06) （2011BAD35B06）
973前期项目(2012CB126311) （2012CB126311）
国家自然基金项目(31201227) （31201227）
中国博士后项目(20110491024) （20110491024）
黑龙江省博士后项目(LBH11220) （LBH11220）
黑龙江省骨干教师项目的资助项目(1252G014). （1252G014）
