动物学研究2012，Vol.33Issue(5)：473-480,8.DOI:10.3724/SP.J.1141.2012.05473
黄河裸裂尻鱼肌肉生长抑制素基因克隆及表达分析
cDNA cloning and expression analysis of MSTN gene from Schizopygopsis pylzovi
摘要
Abstract
Myostatin (MSTN) is a member of the TGF-β superfamily that acts as a negative regulator of skeletal muscle growth. A full-length, 2180 bp, cDNA sequence of the myostatin gene from Schizopygopisis pylzovi was cloned with RT-PCR,5'-RACE and 3'-RACE and the cDNA clone included a 1128 bp ORF, encoding a 375 amino acid peptide. Using PCR, we obtained the sequences of two introns of the MSTN gene and found that its structure in Schizopygopsis pylzovi was similar to that of other vertebrates, including three exons and two introns. Likewise, the putative MSTN peptide of Schizopygopsis pylzovi contains a conserved RXXR proteolytic cleavage domain, and 8 conserved cysteine residues in the C. terminal of the protein, similar to other vertebrates. Phylogenetic analysis showed that the MSTN of Schizopygopsis pylzovi has high homology with other cyprinid fishes, but a low homology with mammals and birds. In the 9 examined tissues, the MSTN gene was highly expressed in heart, kidney, intestine and spermary, while weakly expressed in muscle, brain, fat, gill and hepatopancreas. Quantitative real-time PCR analysis showed that the expression of MSTN gene was different during embryo development, suggesting that the fish MSTN may not only play roles in muscle development but also contribute to other biological functions.关键词
黄河裸裂尻鱼/肌肉生长抑制素/基因克隆/表达Key words
Schizopygopsispylzovi/ Myostatin/ Gene cloning/ Expression分类
生物科学引用本文复制引用
晁燕,张辉,祁得林,赵兰英,李长忠,谢保胜,申志新,王国杰,王振刚,李超,拜彬强..黄河裸裂尻鱼肌肉生长抑制素基因克隆及表达分析[J].动物学研究,2012,33(5):473-480,8.基金项目
国家自然科学基金项目(30860048,31160226) （30860048,31160226）
教育部新世纪优秀人才支持计划(NCET-09-0861) （NCET-09-0861）
