动物营养学报2012，Vol.24Issue(11)：2154-2164,11.DOI:10.3969/j.issn.1006-267x.2012.11.013
饲料脂肪水平对吉富罗非鱼生长性能及其在低温应激下血清生化指标和肝脏脂肪酸组成的影响
Effects of Dietary Lipid Level on Growth Performance of Genetic Improvement of Farmed Tilapia (GIFT, Oreochromis niloticus) and Its Serum Biochemical Indices and Fatty Acid Composition under Cold Stress
摘要
Abstract
The objective of this study was to evaluate the effects of dietary lipid level on growth performance of genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus) and its serum biochemical indices and fatty acid composition under cold stress. Fish with an average body weight of (37. 0 ± 1. 0) g were fed experimental diets containing 4 different levels (1. 95% , 6. 19% , 8. 03% and 9. 95% , respectively) of lipid which were supplemented with 0, 4% , 6% and 8% of soybean oil in basal diet (1. 95% lipid) for 8 weeks. Each treatment contained 3 replicates and each replicate contained 25 fish. After feeding experiment, experimental fish were acclimated at 28 ℃ as control, the water temperature was reduced from 28 ℃ to 15 ℃ at a rate of 1 t/h to proceed cold stress experiment. The results showed as follows; 1) the survival rate (SR) and feed conservation rate (FCR) were not significantly influenced by dietary lipid level (P > 0. 05). The weigh gain rate (WGR) and specific growth rate (SGR) increased significantly (P <0. 05) with dietary lipid level increasing until dietary lipid level arrived at 6. 19% . 2) Under the cold stress, dietary lipid level had significant influence on serum biochemical indices except glucose (GLU) and triglyceride (TG) levels (P <0. 05). With dietary lipid level increasing, the levels of GLU, total protein (TP) , TG, cholesterol (CHOL) and low density lipoprotein cholesterol (LDL-C) in serum gradually raised, and the serum high density lipoprotein cholesterol (HDL-C) level showed a rising trend and then gradually descended, while the activities of aspartate aminotransferase (AST) and alanine transarninase (ALT) in serum slightly decreased and then increased. 3) Under the cold stress, the proportion of saturated fatty acid (SFA) in liver showed a descending trend and the proportion of unsaturated fatty acid (UFA) in liver showed a rising trend with dietary lipid level increasing. Dietary lipid level had significant influence on n-3 polyunsaturated fatty acid (PUFA) proportion, n-6 PUFA proportion and n-3/n-6 (P <0. 05). In conclusion, the optimal level of dietary lipid is 6.19% , which is beneficial to promote the growth of GIFT and its cold-resistance ability. When dietary lipid level reaches or exceeds 8.03% , the higher transaminase activity may burden the metabolism of liver, which is bad for health and growth offish. [ Chinese Journal of Animal Nutrition, 2012, 24(11) :2154-2164]关键词
吉富罗非鱼/饲料脂肪水平/生长性能/低温应激/血清生化指标/脂肪酸组成Key words
GIFT/ dietary lipid level/ growth performance/ cold stress/ serum biochemical indices/ fatty acid composition分类
农业科技引用本文复制引用
石桂城,董晓慧,陈刚,谭北平,迟淑艳,杨奇慧,刘泓宇..饲料脂肪水平对吉富罗非鱼生长性能及其在低温应激下血清生化指标和肝脏脂肪酸组成的影响[J].动物营养学报,2012,24(11):2154-2164,11.基金项目
广东省自然科学基金(S2011010005892)及国家支撑计划(2008BADB9B01) （S2011010005892）
