动物营养学报2012，Vol.24Issue(11)：2237-2242,6.DOI:10.3969/j.issn.1006-267x.2012.11.023
断奶应激对仔猪肠形态、肠黏膜屏障和p38丝裂原活化蛋白激酶信号通路的影响
Effects of Weaning Stress on Intestinal Morphology, Mucosal Barrier and p38 MAPK Signaling Pathway in Piglets
摘要
Abstract
This experiment was conducted to investigate the effects of early weaning stress on intestinal morphology , mucosal barrier and p38 mitogen-activated protein kinase (p38 MAPK) signaling pathway of piglets. Piglets (Duroc x Landrace x Yorkshire) of 12 litters were chosen and 2 to 3 piglets (adjusted for body weight) from each litter were weaned and served as a weaned group. The remaining unweaned piglets were continued to nurse and served as an unweaned group. Six piglets of each group were chosen to slaughter at 22, 24, 28 and 35 days of age, respectively. The results showed as follows; compared with the unweaned group, jejunal villus height and the ratio of villus height to crypt depth at 22 , 24 and 28 days of age in the weaned group were decreased (P < 0. 05) , while there was no significant difference at 35 days of age (P > 0. 05). The ratio of villus height to crypt depth of the weaner piglets at 24 and 28 days of age was lower than that at 22 days of age (P <0. 05). The weaner piglets had higher jejunal villus height and the ratio of villus height to crypt depth at 35 days of age than those at 22, 24 and 28 days of age (P < 0. 05). Compared with the unweaned group, plasma D-lactate content and diamine oxidase activity were increased with the increasing duration of weaning and still higher than those in the weaned group at 35 days of age (P <0. 05). Weaning stress activated p38 MAPK signaling pathway and significantly increased the ratio of colonic mucosa phospho-p38 MAPK and total p38 MAPK expression at 24 days of age compared with 22 days of age (P <0. 05). The results indicate that early weaning induces the damage of intestinal morphology and mucosal barrier. Although damaged intestinal morphology is near to reinstitution entirely at 35 days of ages, intestinal mucosal barrier is not recovered, indicating the recovery of mucosal barrier is slower than that of morphology. Early weaning stress activates p38 MAPK signaling pathway. [ Chinese Journal of Animal Nutrition, 2012, 24 (11) : 2237-2242 ]关键词
断奶仔猪/肠形态/肠通透性/p38丝裂原活化蛋白激酶信号通路Key words
weaner piglets/ intestinal morphology/ intestinal permeability/ p38 MAPK signaling pathway分类
农业科技引用本文复制引用
栾兆双,姚丽丽,傅振宁,宋娟,胡彩虹..断奶应激对仔猪肠形态、肠黏膜屏障和p38丝裂原活化蛋白激酶信号通路的影响[J].动物营养学报,2012,24(11):2237-2242,6.基金项目
国家自然科学基金(31072039) （31072039）
浙江省自然科学基金(Z3100071) （Z3100071）
农业部948(2011-G7) （2011-G7）
中央高校基本科研业务费专项资金(2011FZA6018) （2011FZA6018）
教育部长江学者和创新团队发展计划(IRT1040) （IRT1040）
