南方医科大学学报2012，Vol.32Issue(11)：1559-1563,5.DOI:10.3969/j.issn.1673-4254.2012.11.006
下调miR-21可以增强食管癌TE-1细胞的放射敏感性
Down-regulation of miR-21 expression enhances the radiosensitivity of TE-1 cells in vitro
摘要
Abstract
Objective To study the effect of miR-21 down-regulation on the radiosensitivity of TE-1 cells in vitro. Methods TE-1 cells were transfected via lentivirus with a vector containing the antisense oligonucleotides of miR21, and the subclones with stable down-regulation of miR21 expression were selected with puromycin and designated as TE-l-miR21-, whose expression level of miR21 was determined using real-time quantitative PCR. The radiosensitivity of TE-1 and TE-l-miR21 cells were evaluated with colony formation assay, and the expressions of β-catenin was determined using Western blotting and RT-PCR. Flow cytometry was used to analyze the proportion of p75NTR+ cells in TE-1 and TE-l-rmR21- cells. Results A cell subclone stably expressing a low level of miR21 was obtained and verified by real-time quantitative PCR. Colony formation assay showed an enhanced the radiosensitivity of TE-l-miR21- cells compared to parental TE-1 cells. RT-PCR revealed no significant changes in β-catenin mRNA expression in TE-l-miR21- cells, whereas its β-catenin protein expression was markedly suppressed by high-dose (8 and 10 Gy) irradiation. Flow cytometry assay showed a decreased proportion of p75NTR+ cells in TE-l-miR21- cells compared to that in TE-1 cells. Conclusion Down-regulation of miR21 can enhance the radiosensitivity of TE-1 cells, which might result from the inactivation of wnt/β-catenin signal pathway and a decreased p75NTR+ cell proportion.关键词
食管癌/放射敏感性/miR-21/Wnt/β-catenin信号通路/p75NTRKey words
esophageal cancer/ radiosensitivity/ miR-21/ wnt/β-catenin pathway/ p75NTR分类
医药卫生引用本文复制引用
李晓青,陈鑫,黄珊,车少敏,张晓智..下调miR-21可以增强食管癌TE-1细胞的放射敏感性[J].南方医科大学学报,2012,32(11):1559-1563,5.基金项目
国家自然科学基金(30972962) （30972962）
