南方医科大学学报2012，Vol.32Issue(11)：1597-1599,3.DOI:10.3969/j.issn.1673-4254.2012.11.015
白头翁汤对溃疡性结肠炎小鼠肠道miR-19a表达的影响
Effect of Baitouweng Decoction on intestinal miR-19a expression in mice with ulcerative colitis
摘要
Abstract
Objective To investigate the therapeutic effect of Baitouweng Decoction on dextran sodium sulphate (DSS)-induced ulcerative colitis in mice and explore its mechanism involving miR-19a. Methods Forty female c57 mice were randomly allocated into 4 equal groups, namely the normal control group, model group (treated with 3.5% DSS solution), treatment group (treated with DSS+Baitouweng Decoction), and positive control group (treated with DSS+5-ASA). Ulcerative colitis was induced in the mice by feeding them with 3.5% DSS in drinking water, and the mice in the control group were given water only. The disease activity index (DAI) of the mice in each group was recorded daily. Seven days later, the mice were sacrificed for histological examination of the intestines using HE staining; the expression of miR-19a mRNA in the intestines was detected using RT-qPCR. Results Compared with the control group, the model group showed significantly increased DAI and histological scores, and administration of Baitouweng Decoction significantly lowered the DAI and histological scores of the DSS-treated mice. The expression of miR-19a was lowered following DSS treatment, and Baitouweng Decoction treatment caused an increased miR-19a expression in DSS-treated mice. Conclusion Baitouweng Decoction has therapeutic effects on DSS-induced ulcerative colitis in mice, and this effect is probably mediated by enhancement of miR-19a expression in the intestines.关键词
白头翁汤/溃疡性结肠炎/miR-19aKey words
Baitouweng Decoction/ ulcerative colitis/ miR-19a分类
医药卫生引用本文复制引用
周鹏志,刘凤斌,罗琦,孙嫣,丁飞跃,陈斌..白头翁汤对溃疡性结肠炎小鼠肠道miR-19a表达的影响[J].南方医科大学学报,2012,32(11):1597-1599,3.基金项目
国家自然科学基金青年科学基金(81001506) （81001506）
广东省中医药局科研课题计划(20112157) （20112157）
广州中医药大学校级创新基金(10CX002) （10CX002）
