南方医科大学学报2012，Vol.32Issue(11)：1651-1654,4.DOI:10.3969/j.issn.1673-4254.2012.11.029
外源性腐胺对正常大鼠肾功能和细胞凋亡的影响
Exogenous putrescine causes renal function impairment and cell apoptosis in rats
摘要
Abstract
Objective To explore the effect of exogenous putrescine on renal function and cell apoptosis in rats. Methods Ninety SD rats were randomized into control group (n=W), high-dose putrescine group (P1 group, n=40), and low-dose putrescine group (P2 group, n=40) with intraperitoneal injections of 2 ml of normal saline, 50 μg/g putrescine, and 25 μg/g putrescine, respectively. At 24, 48, 72 and 96 h after the injections, 10 rats from each group were sacrificed to examine serum Cr and BUN levels, histological changes in the kidneys, and renal cell apoptosis (TUNEL assay). Results The rats in the two putrescine-treated groups showed mild edema in some renal tissues without obvious necrosis. In PI and P2 groups, serum Cr and BUN levels differed significantly at each time point of measurement (P<0.01 and P<0.05, respectively), and were significantly higher than the levels in the control group (P<0.01 and P<0.05, respectively). The two putrescine-treated groups showed gradually increased renal cell apoptosis with time, reaching the peak levels at 96 h and 48 h, respectively. The peak renal cell apoptosis rates in P1 [(24.78±2.19)%] and P2 [(26.27±2.13)%] group were significantly higher than the rate in the control group [(4.47± 0.33)%, P<0.01]. Conclusion Exogenous putrescine can lead to renal function impairment and induce renal cell apoptosis in rats, and the severity of these changes appeared to be associated with the blood concentration of exogenous putrescine.关键词
腐胺/肾功能/细胞凋亡/大鼠Key words
putrescine/ renal function/ cell apoptosis分类
医药卫生引用本文复制引用
周岳平,肖能坎,荣新洲,樊桂成,刘思容..外源性腐胺对正常大鼠肾功能和细胞凋亡的影响[J].南方医科大学学报,2012,32(11):1651-1654,4.基金项目
广东省医学科研基金(2010-A454) （2010-A454）
