南方医科大学学报2012，Vol.32Issue(11)：1675-1679,5.DOI:10.3969/j.issn.1673-4254.2012.11.035
内质网应激相关分子GRP78在高血压加重全脑缺血大鼠神经损伤中的作用
Role of endoplasmic reticulum stress-related molecule GRP78 in global cerebral ischemia-reperfusion injury aggravated by hypertension in rats
摘要
Abstract
Objective To observe the changes in the expression of glucose-regulated protein 78 (GRP78) in the hippocampus of normal rats and spontaneously hypertensive rats with cerebral ischemia/reperfusion (IR) and explore the possible role of GRP78 in global cerebral IR injury aggravated by hypertension. Methods Sixty male Wistar-Kyoto (WKY) rats were randomly divided into sham operation group and global cerebral ischemia-reperfusion group (I/R) and a Another 30 male rats with spontaneous hypertension served as hypertensive cerebral ischemia and reperfusion (SHR + I/R) group. Global cerebral ischemia model was established in rats using a modified four-vessel occlusion method. The morphological changes of the neurons in the hippocampal region were observed using HE staining, and GRP78 expression was detected using immunohistochemistry and Western blotting. The behavioral changes of the rats were examined using the Eight-arm Maze. Results Compared with the rats with sham operation, the rats in I/R group showed a decreased density of surviving neurons in the hippocampus and an increased expression of GRP78, which reached the peak level at 24 h. Compared with that in I/R group, the density of surviving neurons decreased obviously at each time point in SHR + IR group, and GRP78 expression significantly increased at 6 h, followed then by progressive reduction at 24 and 48 h. The rats in SHR+IR group and I/R group showed no significant difference in behavior changes. Conclusion Hypertension can aggravate global cerebral IR injury by decreasing GRP78 expression and increasing the loss of nerve cells.关键词
高血压/脑缺血再灌注/葡萄糖调节蛋白78/内质网应激Key words
hypertension/ cerebralischemia and reperfusion/ glucose-regulated protein78/ endoplasmic reticulum stress分类
医药卫生引用本文复制引用
赵雅宁,李建民,刘乐,常学优,陈长香,李淑杏..内质网应激相关分子GRP78在高血压加重全脑缺血大鼠神经损伤中的作用[J].南方医科大学学报,2012,32(11):1675-1679,5.基金项目
河北省自然科学基金(H2012401007) （H2012401007）
河北省教育厅重点课题(ZD2010106) （ZD2010106）
