福建农业学报2012，Vol.27Issue(10)：1032-1038,7.
变异型PRRSV-FJ09A毒株ORF5和NSP2基因的遗传变异分析及致病性
Genetic Variations of ORF5, NSP2 of Variant PRRSV-FJ09A strain and It's Pathogenicity
摘要
Abstract
To fully understand the knowledge of variant porcine reproductive and respiratory syndrome virus (PRRSV) genetic variation and pathogenicity, the ORF5 and NSP2 genomes of PRRSV-FJ09A strain were amplified by RT-PCR and sequenced. Comparing with PRRSV-VR2332, ORF5 gene shared 87. 6% of nucleotide homologies and 88. 1 % of the deduced amino acid homologies, there were point mutations within deduced amino acids, focus on amino acids of associated with virulence, epitope and N-glycosylation sites; NSP2 gene shared 80. 9% of nucleotide homologies and 77. 0% of the deduced amino acid homologies, and it shared a common feature of 31 amino acids deletion in three places which one place more of a phenylalanine (F) than other local reports. Comparing these sequences with Chinese PRRSV strains published after 2006, ORF5 gene shared 98. 5 % - 99. 7% of nucleotide homologies and 97. 5% - 99. 5% of the deduced amino acid homologies, NSP2 gene shared 98. 4% - 99. 3% of nucleotide homologies and 97. 3% - 98. 9% of the deduced amino acid homologies. The phylogeneic tree revealed PRRSV-FJ09A strain isolated had closer relationship with PRRSV-FJ06A strain. According to the animal experiment PRRSV-FJ09A had a high pathogenicity to 30 d pigs.关键词
变异型繁殖与呼吸综合征/ORF5基因/NSP2基因/遗传变异/致病性Key words
variant porcine reproductive and respiratory syndrome/ ORF5 gene/ NSP2 gene/ genetic variations/pathogenicity分类
农业科技引用本文复制引用
王隆柏,张志灯,车勇良,吴学敏,陈如敬,邵良平,庄向生,周伦江..变异型PRRSV-FJ09A毒株ORF5和NSP2基因的遗传变异分析及致病性[J].福建农业学报,2012,27(10):1032-1038,7.基金项目
福建省农业科学院科技计划项目(B2009-01) （B2009-01）
福建省农业科学院科技下乡"双百"行动项目(sbmx1202) （sbmx1202）
