福建农业学报2012，Vol.27Issue(10)：1044-1049,6.
2个水稻器官特异表达基因的鉴定及分析
Identification and Analysis of Two Organ-specific Expressed Genes in Rice
摘要
Abstract
Research on tissue-specific expressed genes is essential for the understanding of the molecular mechanism of plant growth and development. By means of bioinformatics, RT-PCR and real-time PCR, two tissue-specific expressed genes in rice, Os01g18840 and Os01g28500, were identified. The OsO1g-18840 gene was specially expressed in the leaves, and Os01g28500 in the stems, leaves and glumes. Furthermore, under the stress treatments of PEG, NaCl, ABA or in the dark, the expression of the Os01g18840 gene was reduced, but that of the Os01g28500 gene significantly induced. Analyses on the structures of the genes and proteins revealed that the Os01g18840 gene encoded an intermediate filament protein containing one coiled-coil domain, while the Os01g28500 protein had a SCP-like domain relating to pathogenic infection. In addition, the analysis on their upstream promoter region indicated that there were a large number of cis-elements relating to leaf, mesophyll cell or green tissue expression.关键词
器官特异表达/水稻/基因/顺式元件Key words
Organ-specific expression/ rice (Oryza sativa L. ) / gene/ cis-element分类
生物科学引用本文复制引用
周淑芬,刘华清,林智敏,颜静宛,赵庆欣,陈睿,杨绍华,王锋..2个水稻器官特异表达基因的鉴定及分析[J].福建农业学报,2012,27(10):1044-1049,6.基金项目
福建省自然科学基金项目(2009J01091) （2009J01091）
福建省财政专项——福建省农业科学院科技创新团队建设项目(CXTD2011-09) （CXTD2011-09）
