分析测试学报2012，Vol.31Issue(12)：1505-1512,8.DOI:10.3969/j.issn.1004-4957.2012.12.006
石墨烯-壳聚糖复合物修饰电极构建电化学免疫传感器对1-芘丁酸的检测研究
An Electrochemical Immunosensor for PBA Detection Based on Graphene-Chitosan Composite Film Modified Electrode
摘要
Abstract
An highly sensitive, stable and label-free electrochemical immunosensor for the detection of 1 -pyrenebutyric acid (PBA) was developed based on graphene (GS) and chitosan (CS) composites modified glassy carbon electrode ( GS - CS/GCE ) . The proposed GS - CS/GCE was activated by the l-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride ( EDC ) and N-hydroxysuccinimide (NHS) (4:1) solution in order to covalently immobilize PAHs antibody (anti-PAHs). The morphology of the GS - CS composites film was characterized by transmission electron microscopy (TEM). In phosphate buffer solution (pH 7. 0) containing 0. 1 mol/L KC1 and 10 mmol/L K3Fe( CN)6 solution (0. 1 mol/L KC1 + 10 mmol/L K3Fe(CN)6 +0. 1 mol/L PBS(pH 7. 0)), the electrochemical behaviors of the modified electrode and the electrochemical properties of the immunosensor were studied by cyclic voltammetry ( CV) and differential pulse voltammetry ( DPV). Due to the synergistic effect of grapheme and chitosan, the peak current of GS - CS/GCE in the supporting electrolyte was largely increased, which was attributed to improve sensitivity of the immunosensor. Under the optimized conditions, the immobilization amount of antibody was significantly improved on the modified e-lectrode, resulting in amplifying the molecular recognition ability of the modified electrodes. Because of the poor conductivity of the antybody - antigen immunocomplex, the current response of the proposed immunosensor decreased linearly with increase of PBA concentration. The linear range of the immunosensor for PBA detection was 0. 1 -80 μg/L with a detection limit of 0. 03 μg/L. With excellent selectivity and reproducibility, the proposed immunosensor was applied in the determination of PBA in the real sample with spiked recoveries of 90% -105% .关键词
电化学免疫传感器/1-芘丁酸(PBA)/石墨烯/壳聚糖Key words
electrochemical immunosensor/ 1-pyrenebutyric acid (PBA) / graphene/ chitosan分类
化学化工引用本文复制引用
许双姐,吴根英,许贺,柳建设,金利通..石墨烯-壳聚糖复合物修饰电极构建电化学免疫传感器对1-芘丁酸的检测研究[J].分析测试学报,2012,31(12):1505-1512,8.基金项目
国家自然科学基金青年基金(21005014) （21005014）
上海市教育发展委员会"晨光计划"资助项目(11CG34) （11CG34）
