分析测试学报2012，Vol.31Issue(12)：1536-1539,4.DOI:10.3969/j.issn.1004-4957.2012.12.011
纤维素键合手性固定相拆分喹禾灵对映体
Enantiomeric Resolution of Quizalofop-ethyl on Immobilized Cellulose Chiral Stationary Phase
摘要
Abstract
The enantiomeric resolution of quizalofop-ethyl on Chiralpak IC column ( cellulose — tris( 3 , 5-dichlorophenylcarbamate) immonilized on silica) was studied. Effects of mobile phase composition, flow rate and temperature on the chiral separation of enantiomers were investigated. The result showed that a good baseline separation of quizalofop-ethyl enantiomers was obtained by using n-hexane - isopropanol(95 : 5) as mobile phase with a flow rate of 1. 0 mL/min at 25 ℃. The resolution was up to 3.97, in which R-enantiomer was firstly eluted. The limits of detection ( LOD) for both of enantiomers were 0.045 mg/L and the limits of quantitation ( LOQ) were 0. 15 mg/L. The Van't Hoff plot of the lnα versus 1/T showed a good linearity with correlation coefficients higher than 0.99 at column temperature of 15 -35 ℃. The resolution factor was decreased with the increase of column temperature. The enthalpy and entropy change difference showed that the enantioseparation of quizalofop-ethyl enantiomers was enthalpy driven.关键词
喹禾灵/手性拆分/Chiralpak IC柱/高效液相色谱Key words
quizalofop-ethyl/enantioseparation/Chiralpak IC column/HPLC分类
化学化工引用本文复制引用
袁庆东,沈国强,张晓天,江乾,王保国,张金利,李韡..纤维素键合手性固定相拆分喹禾灵对映体[J].分析测试学报,2012,31(12):1536-1539,4.基金项目
国家自然科学基金资助项目(NSFC 20836005) （NSFC 20836005）
教育部博士点基金资助项目 （）
