法医学杂志2012，Vol.28Issue(6)：408-412,5.DOI:10.3969/j.issn.1004-5619.2012.06.002
混合人血清诱发豚鼠过敏反应死亡模型的改良
The Improvement of Mixed Human Serum-induced Anaphylactic Reaction Death Model in Guinea Pigs
陈炯垣 1赖跃 1李冬日 2岳霞 1王慧君1
作者信息
- 1. 南方医科大学法医系,广东广州510515
- 2. 深圳市公安局刑事科学技术研究所,广东深圳518040
- 折叠
摘要
Abstract
Objective To increase the death rate of fatal anaphylaxis in guinea pigs and the detectable level of the tryptase of mast cell in blood serum. Methods Seventy-four guinea pigs were randomly divided into five groups: original model group, original model control group, improved model group, improved model control group, improved model with non-anaphylaxis group. Using mixed human serum as the allergen, the way of injection, sensitization and induction were improved. ELISA was used to detect the serum mast cell tryptase and total IgE in guinea pigs of each group. Results The death rate of fatal anaphylaxis in original model group was 54.2% with the different degree of hemopericardium. The severe pericardial tamponade appeared in 9 guinea pigs in original model group and original model control group. The death rate of fatal anaphylaxis in improved model group was 75% without pericardial tamponade. The concentration of the serum total IgE showed no statistically difference between original model group and original model control group (P>0.05), but the serum mast cell tryptase level was higher in the original model group than that in the original model control group (P<0.05). The concentration of the serum total IgE and the serum mast cell tryptase level were significantly higher in improved model group than that in the improved model control group (P<0.05). Conclusion The death rate of the improved model significantly increases, which can provide effective animal model for the study of serum total IgE and mast cell tryptase.关键词
法医病理学/过敏反应/模型,动物/豚鼠Key words
forensic pathology/ anaphylaxis/ models, animal/ guinea pigs分类
社会科学引用本文复制引用
陈炯垣,赖跃,李冬日,岳霞,王慧君..混合人血清诱发豚鼠过敏反应死亡模型的改良[J].法医学杂志,2012,28(6):408-412,5.
