法医学杂志2012，Vol.28Issue(6)：445-447,3.DOI:10.3969/j.issn.1004-5619.2012.06.012
二核苷酸STR基因座D6S261的多态性检测
Genetic Polymorphisms of the Dinucleotide STR Locus D6S261
摘要
Abstract
Objective To investigate the application of dinucleotide STR locus in paternity testing. Methods Dinucleotide STR locus D6S261 was selected and the paternity testing blood samples were amplified using 200 random blood samples, 16 family samples and 193 paternity test samples. Data of the PCR products were collected by 3130XL Genetic Analyzer and the genetic parameters of population were calculated by PowerStats v12. Results Fifteen alleles and 50 genotypes were found and H, DP, PE and PIC were 0.850, 0.953, 0.695, and 0.820, respectively. The typing results of both family samples and paternity test samples were accord with the law of inheritance, which no mutation was discovered. Conclusion The genetic polymorphisms of D6S261 show good characteristics with low mutation rate and high stability. It can be an effective method to solve the indetermination caused by mutation in paternity testing if the stutter bands can be decreased.关键词
法医遗传学/多态现象,遗传/二核苷酸/亲子鉴定Key words
forensic genetics/ polymorphism, genetic/ dinucleotide/ paternity testing分类
社会科学引用本文复制引用
杨鹏,刘亚楠,聂燕钗,周怀谷,赵子琴..二核苷酸STR基因座D6S261的多态性检测[J].法医学杂志,2012,28(6):445-447,3.基金项目
上海市科学技术委员会科研计划项目(09231203600) （09231203600）
