高等学校化学学报2012，Vol.33Issue(12)：2827-2832,6.DOI:10.7503/cjcu20120006
静电纺丝法制备PBS纳米纤维膜及缓释血小板内生长因子的PBS纳米纤维膜的生物活性评价
Preparation and Bioactivities of Electrospun PBS Nanofiber Membrane and Hybrid PBS Nanofiber Membrane of Sustained Release Growth Factors in Platelet
摘要
Abstract
Poly(butylene succinate) (PBS) nanofiber membrane and hybrid nanofiber membrane of PBS and platelet-rich plasma(PRP) were prepared by electrospinning. The properties and bioactivities of scaffold materials were evaluated by means of scanning electron microscope ( SEM) , methyl thiazolyl tetrazolium ( MIT) , fluorescent staining and laser scanning co-focal microscope, hemolytic properties of materials and enzyme-linked immunosorbent assay(ELISA). The experiment results show that nanofiber membranes can accelerate the proliferation of human osteosarcoma MG63 cells line. Also, in vitro the growth factors in the scaffold material display a sustained release with the scaffold degrading after the initial burst release. In addition, hemolytic rate of scaffold material is 0. 1475% ( <5% ) , which meets the demand of hemolytic rate of medial materials.关键词
纳米纤维膜/聚丁二酸丁二醇酯/生长因子/富血小板血浆/缓释Key words
Nanofiber membrane/ Poly(butylene succinate)/ Growth factor/ Platelet-rich plasma/ Sustained release分类
化学化工引用本文复制引用
孙淑芬,李男男,刘敏,刘译聪,张英超,周延民..静电纺丝法制备PBS纳米纤维膜及缓释血小板内生长因子的PBS纳米纤维膜的生物活性评价[J].高等学校化学学报,2012,33(12):2827-2832,6.基金项目
国家自然科学基金(批准号:81070855)资助. （批准号:81070855）
