肝胆胰外科杂志2012，Vol.24Issue(6)：470-473,4.
Notch信号通路在小鼠肝癌细胞H22增殖凋亡中的作用及意义
The role and significance of Notch signaling pathway in the proliferation and apoptosis of H22 cells in mice
摘要
Abstract
Objective To observe the role of Notch signaling pathway in the proliferation and apoptosis of mouse hepatocellular carcinoma cell line H22, and to explore its possible mechanism. Methods Cultured H22 cells were randomly divided into A, B and C group, Notch signaling activator rhNF-KB, Notch signaling inhibitor DAFT, and PBS, were added separately into above three groups, then cultured together for 48 h. Cell proliferation was observed with MTT method (cell absorbance value), cell apoptosis was detected with flow cytometry method, and the treated cells were analyzed with immunohistochemistry method (fluorescence staining). Notch1, Hes1, and Bcll-2 mRNA and the corresponding proteins in cultured cells were detected with RT-PCR and Western blotting methods, respectively. Results After 24, 48, 72 and 96 h of cell culture, the difference of cell proliferation among A, B, C group was significant (all P <0.05). The apoptosis rates of A, B, C group were 25.52% ± 3.13%, 0.91% ± 0.18%, 8.91% ± 1.50%, respectively, the difference among three groups was significant (all P<0.05). Compared with group C, Notch1, Hes1, Bcl~2 mRNA and their protein expression in group A were significantly increased (P<0.05), whereas the Notch1, Hes1, Bcl~2 mRNA and their protein expression in group B were significantly decreased (P<0.05). Conclusion Notch signaling pathway plays an important role in the inhibition of cell proliferation and the promotion of cell apoptosis of mouse hepatocellular carcinoma cell line H22. The possible mechanism may be related to its regulation of the expression of target gene Hes1 and Bcl-2.关键词
Notch信号通路/Hes1基因/Bcl-2基因/细胞凋亡/细胞增殖/肝癌细胞H22/小鼠Key words
signaling pathway/ Hesl gene/ Bcl-2 gene/ cell apoptosis/ cell proliferation/ hepatocellular carcinoma cell line H22/ mice分类
医药卫生引用本文复制引用
陈勇,金晓燕,梁勇,杨林军..Notch信号通路在小鼠肝癌细胞H22增殖凋亡中的作用及意义[J].肝胆胰外科杂志,2012,24(6):470-473,4.基金项目
2010年国家自然科学基金项目(81072209) （81072209）
2010年台州市科技项目(102ky11) （102ky11）
2010年浙江省台州市椒江区科技项目(10278). （10278）
