干旱地区农业研究2012，Vol.30Issue(6)：23-31,9.
芸芥自交亲和性的遗传及自交亲和基因mRNA差异显示分析
Analysis of self-compatibility genes by mRNA differential display technique and inheritance of self-compatibility in E.sativa Mill
摘要
Abstract
18 hybridized combinations were made between 8 self-compatible lines and 4 self-incompatible lines, and inheritance of self-compatibility in E. Sativa Mill was analysed. Furthermore, expresstion tissue of self-compatible genes was preliminary analysed, and specific cDNA fragments related to self-compatibility were isolated and screened by using mRNA differential display technique. The results showed that all the F1 plants were self-incompatible, and the F2 and BC1 population segregated in ratio. Through chi square test, the segregation ratios of self-incompatibility to self-compatibility in F2 and BC| population were coincided with the ratio of 1:3 and 1:1, respectively. Therefore, it was inferred that the hereditary of self-compatibility in Yunjie was controlled by one pair recessive nucleus gene. Self-compatible genes of Yunjie was not constitutive expression, but that belonged to specific tissue expression. Self-compatible lines had 3 differentially expressed bands which exhibited stably with different primers, these cDNA bands were closely related to self-compatibility of Yunjie.关键词
芸芥/自交亲和性的遗传/自交亲和基因/mRNA差异显示技术Key words
E. Sativa Mill/ inheritance of self-compatibility/ self-compatible genes/ mRNA differential display分类
农业科技引用本文复制引用
范惠玲,白生文,张芬琴,肖占文,陈修斌,孙万仓..芸芥自交亲和性的遗传及自交亲和基因mRNA差异显示分析[J].干旱地区农业研究,2012,30(6):23-31,9.基金项目
国家863计划资助项目(2009AA101105) （2009AA101105）
河西学院青年教师科研基金项目(QN2011-17) （QN2011-17）
