干旱地区农业研究2012，Vol.30Issue(6)：128-133,6.
外源NO对渗透胁迫下黑麦草种子萌发及活性氧代谢的影响
Effects of exogenous nitric oxide on seed germination and reactive oxygen species metabolism of seedling leaves in ryegrass under osmotic stress
摘要
Abstract
To discuss the regulating effect of exogenous nitric oxide (NO) on the physiological response of herbage under osmotic stress of simulated by polyethylene glycol, we investigated the effects of exogenous NO donor sodium nitro-prusside (SNP) on the seed germination, reactive oxygen species metabolism and osmotica accumulation in seedling leaves with the experimental materials of ryegrass( Lolium perenne L) . The results showed that under the stress, applying 100 μmol/L SNP increased significantly the seed germination rate, germination index, and vigor index, alleviated the decreases of fresh and dry weights of whole plant, increased the activities of superoxide dismutase (SOD) , guaiacol peroxi-dase (POD) , ascorbate peroxidase (APX) and plasma membrane H+ — ATPase and the contents of glutathione, soluble sugar, free amino acid, proline, K+ , and Na+ as well as plant water content, and decreased O2- production rate and the contents of H2O2, malondialdehyde and soluble protein in leaves. The results indicated that exogenous NO could promote the seed germination of ryegrass under osmotic stress and alleviate the membrane lipid peroxidation induced by osmotic stress in seedling leaves.关键词
渗透胁迫/一氧化氮/种子萌发/活性氧/渗透溶质/黑麦草Key words
osmotic stress/ nitric oxide/ seed germination/ reactive oxygen species/ osmotica/ ryegrass分类
农业科技引用本文复制引用
刘建新,王金成,贾海燕,王凤琴..外源NO对渗透胁迫下黑麦草种子萌发及活性氧代谢的影响[J].干旱地区农业研究,2012,30(6):128-133,6.基金项目
甘肃省庆阳市科技支撑计划项目(GK098-1-34) （GK098-1-34）
