干旱地区农业研究2012,Vol.30Issue(6):157-161,5.
大豆根瘤菌抗性菌株的筛选及鉴定
Screening and identification of the root nodule bacteria resistant stain associated with soybean
摘要
Abstract
32 rhizobial strains were isolated from well-growth root nodule of soybean from Xi' an and Yangling in Shaanxi. 11 strains were chosen by morphologic method, then resistance test of the strains was made on 5 organic reagents to study the antagonistic property and the phylogeny of the strains. The result showed that XR3, YR3, YR6 and YR7 had good resistance on xylol, phenol, acetone, acrylamideon and fomesafen. And YR3 and YR6 were found to have stronger antagonistic acticity. Based on analysis of the physiological and chemotaxonomic characteristics and 16s rDNA sequence of the 2 strains, the YR3 strain was found to be Rhizobium Leguminosarum U29386, and YR6 strain to be Sinorhizobium Americanum AF506513.关键词
大豆根瘤菌/分离纯化/抗性/鉴定Key words
root nodule bacteria of soybean/ isolating and purifying/ resistance/ identification分类
农业科技引用本文复制引用
郑丹丹,田琴,周丹平,李维平..大豆根瘤菌抗性菌株的筛选及鉴定[J].干旱地区农业研究,2012,30(6):157-161,5.基金项目
国家自然科学基金项目(39670478) (39670478)